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光学相干层析成像(OCT)强度和相位波动与中枢神经系统(CNS)中活动依赖的神经元钙动力学相关[特邀报告]。

OCT intensity and phase fluctuations correlated with activity-dependent neuronal calcium dynamics in the CNS [Invited].

作者信息

Tong Minh Q, Hasan Md Monirul, Lee Sang Soo, Haque Md Rezuanul, Kim Do-Hyoung, Islam Md Shahidul, Adams Michael E, Park B Hyle

机构信息

Graduate Program in Neuroscience, University of California, Riverside, CA 92521, USA.

Department of Bioengineering, University of California, 900 University Ave, Riverside, CA 92521, USA.

出版信息

Biomed Opt Express. 2017 Jan 10;8(2):726-735. doi: 10.1364/BOE.8.000726. eCollection 2017 Feb 1.

Abstract

Phase-resolved OCT and fluorescence microscopy were used simultaneously to examine stereotypic patterns of neural activity in the isolated central nervous system. Both imaging modalities were focused on individually identified bursicon neurons known to be involved in a fixed action pattern initiated by ecdysis-triggering hormone. We observed clear correspondence of OCT intensity, phase fluctuations, and activity-dependent calcium-induced fluorescence.

摘要

相位分辨光学相干断层扫描(OCT)和荧光显微镜被同时用于检查离体中枢神经系统中神经活动的刻板模式。这两种成像方式都聚焦于单个已识别的bursicon神经元,已知这些神经元参与由蜕皮触发激素引发的固定动作模式。我们观察到OCT强度、相位波动与活动依赖性钙诱导荧光之间存在明显的对应关系。

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