用光学相干断层扫描在清醒小鼠中成像局部快速神经激活光学信号。
Imaging localized fast optical signals of neural activation with optical coherence tomography in awake mice.
出版信息
Opt Lett. 2021 Apr 1;46(7):1744-1747. doi: 10.1364/OL.411897.
Abstract
We report optical coherence tomography (OCT) imaging of localized fast optical signals (FOSs) arising from whisker stimulation in awake mice. The activated voxels were identified by fitting the OCT intensity signal time course with a response function over a time scale of a few hundred milliseconds after the whisker stimulation. The significantly activated voxels were shown to be localized to the expected brain region for whisker stimulation. The ability to detect functional stimulus-evoked, depth-resolved FOS with intrinsic contrast from the cortex provides a new tool for neural activity studies.
摘要
我们报告了在清醒小鼠中，由胡须刺激引起的局域快速光学信号（FOS）的光学相干断层扫描（OCT）成像。通过在胡须刺激后几百毫秒的时间尺度内，用响应函数拟合 OCT 强度信号的时程，确定了激活的体素。结果表明，显著激活的体素定位于预期的胡须刺激脑区。利用皮层固有对比度检测功能刺激诱发的、深度分辨的 FOS 的能力为神经活动研究提供了一种新工具。
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