Leong Sze Ying, Burritt David J, Hocquel Alice, Penberthy Ari, Oey Indrawati
Department of Food Science, University of Otago, PO Box 56, Dunedin 9054, New Zealand; Department of Botany, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
Department of Botany, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
Food Chem. 2017 Jul 15;227:404-412. doi: 10.1016/j.foodchem.2017.01.110. Epub 2017 Jan 25.
The present work investigated the bioprotective capacities of red-fleshed sweet cherry cultivars (Prunus avium; Lapins, Stella, Sweetheart and Staccato), with distinct differences in anthocyanins and vitamin C contents, on human intestinal Caco-2 cells exposed to hydrogen peroxide (HO)-induced oxidative stress. Three assays of cell health, the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide cell viability assay, the lactate dehydrogenase membrane leakage assay and nitric oxide production, were used to determine if these cherry digests differed in their ability to protect Caco-2 cells from HO. Cells treated with digests from cherries identified as containing high anthocyanins provided the greatest protection against HO. A strong linear correlation (-0.82<r<0.86) was found between anthocyanin content and cell health biomarkers, indicating that anthocyanins effectively protected Caco-2 cells in a concentration-dependent manner. Sweetheart and Stella cherries demonstrated the greatest bioprotective capacity, suggesting that anthocyanin levels are better markers of a cultivar's ability to protect human cells from oxidative stress than vitamin C.
本研究调查了花青素和维生素C含量存在明显差异的红肉甜樱桃品种（欧洲甜樱桃；拉宾斯、斯特拉、甜心和斯塔卡托）对暴露于过氧化氢（H₂O₂）诱导的氧化应激下的人肠道Caco-2细胞的生物保护能力。采用三种细胞健康检测方法，即3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐细胞活力检测、乳酸脱氢酶膜泄漏检测和一氧化氮生成检测，来确定这些樱桃消化物在保护Caco-2细胞免受H₂O₂损伤的能力上是否存在差异。用被鉴定为含有高花青素的樱桃消化物处理的细胞对H₂O₂提供了最大程度的保护。在花青素含量与细胞健康生物标志物之间发现了很强的线性相关性（-0.82<r<0.86），表明花青素以浓度依赖的方式有效地保护了Caco-2细胞。甜心和斯特拉樱桃表现出最大的生物保护能力，这表明与维生素C相比，花青素水平是一个品种保护人类细胞免受氧化应激能力的更好指标。
