Zhang Xiaoxu, Cheng Zhan, Ma Liyan, Li Jingming
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; Supervision, Inspection & Testing Center for Agricultural Products Quality, Ministry of Agriculture, Beijing, China.
Food Chem. 2017 Jul 15;227:55-63. doi: 10.1016/j.foodchem.2016.12.061. Epub 2016 Dec 22.
Volatile organic compounds (VOCs) and ochratoxin A (OTA) were detected in Aspergillus carbonarius strains (AF, SD26 and SD27) during a 7-day growing period, using HS-SPME-GC-MS and HPLC-MS/MS, respectively. Results showed that 33 VOCs were detected in these strains. The AF strain showed the different sesquiterpene profile compared with the other strains. Principal component analysis revealed that the AF strain was segregated from the other two strains regarding the VOC profile, especially the sesquiterpene profile. Ester metabolites were produced more at the beginning of the strain growth period, whereas extension of the strain incubation resulted in an intense accumulation of hydrocarbon compounds. The prediction models on the correlation between OTA and VOCs were established using partial least squares regression analysis. This analysis indicated that C-8 alcohols, ketones, and trans-nerolidol showed a close relation with the OTA synthesis.
在7天的生长期间，分别使用顶空固相微萃取-气相色谱-质谱联用仪（HS-SPME-GC-MS）和高效液相色谱-串联质谱仪（HPLC-MS/MS），对碳黑曲霉菌株（AF、SD26和SD27）中的挥发性有机化合物（VOCs）和赭曲霉毒素A（OTA）进行了检测。结果表明，在这些菌株中检测到了33种VOCs。与其他菌株相比，AF菌株呈现出不同的倍半萜图谱。主成分分析表明，就VOC图谱而言，尤其是倍半萜图谱，AF菌株与其他两种菌株分离开来。酯类代谢产物在菌株生长初期产生较多，而延长菌株培养时间则导致烃类化合物的大量积累。使用偏最小二乘回归分析建立了OTA与VOCs之间相关性的预测模型。该分析表明，C-8醇、酮类和反式橙花叔醇与OTA合成密切相关。
