Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr el Aini St., P.B. 11562, Cairo, Egypt.
Department of Pharmaceutical Botany, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania; Laboratory of Chromatography, Institute of Advanced Horticulture Research of Transylvania, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania.
Food Chem. 2019 Sep 30;293:233-246. doi: 10.1016/j.foodchem.2019.04.097. Epub 2019 Apr 25.
Ziziphus plants are well recognized for their nutritive and medicinal value worldwide, albeit their chemical profile has yet to be fully reported. The secondary metabolites profile of three traditionally used Ziziphus leaf accessions was investigated via ultra-high performance liquid chromatography coupled to photodiode array and electrospray ionization mass detectors (UHPLC/PDA/ESI-MS). A total of 102 metabolites were characterized revealing the first holistic approach onto Ziziphus leaf metabolome and to include the first report of several novel flavonoids and cyclopeptide alkaloids. Fragmentation pattern for cyclopeptide alkaloids was proposed via ESI-MS. Principal component analysis (PCA) revealed close metabolite resemblance among Z. spina-christi and Z. mauritiana leaf specimens found enriched in saponins and distinct from that of Z. jujuba in which quercetin-3-O-(2-pentosyl)-rhamnoside was most abundant. Further, in-vitro antioxidant, anti-inflammatory and antidiabetic assays revealed for Z. spina-christi and Z. mauritiana strong effects compared to Z. jujuba and in correlation with their metabolites repertoire.
枣属植物因其营养价值和药用价值在全球范围内得到广泛认可，尽管其化学成分尚未得到充分报道。本研究采用超高效液相色谱-光电二极管阵列-电喷雾电离质谱联用技术（UHPLC/PDA/ESI-MS）对三种传统枣叶提取物的次级代谢产物进行了分析。共鉴定出 102 种代谢产物，首次全面研究了枣叶的代谢组学，并首次报道了几种新型黄酮类和环肽生物碱。通过 ESI-MS 提出了环肽生物碱的裂解模式。主成分分析（PCA）表明，在富含皂苷的 Z. spina-christi 和 Z. mauritiana 叶片标本之间存在密切的代谢相似性，与富含槲皮素-3-O-(2-戊糖基)-鼠李糖苷的 Z. jujuba 明显不同。此外，体外抗氧化、抗炎和抗糖尿病测定结果表明，与 Z. jujuba 相比，Z. spina-christi 和 Z. mauritiana 具有更强的作用，这与它们的代谢产物组成有关。
