采用高效液相色谱-二极管阵列检测-质谱联用（HPLC-DAD-MS）和高效液相色谱-光电二极管阵列-（高分辨质谱）-固相萃取-核磁共振联用（HPLC-PDA-(HRMS)-SPE-NMR）技术从枣和酸枣中分离并鉴定环肽生物碱
Isolation and structure elucidation of cyclopeptide alkaloids from Ziziphus nummularia and Ziziphus spina-christi by HPLC-DAD-MS and HPLC-PDA-(HRMS)-SPE-NMR.
作者信息
Tuenter Emmy, Foubert Kenn, Staerk Dan, Apers Sandra, Pieters Luc
机构信息
Natural Products & Food Research and Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
Natural Products & Food Research and Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium.
出版信息
Phytochemistry. 2017 Jun;138:163-169. doi: 10.1016/j.phytochem.2017.02.029. Epub 2017 Mar 8.
Seven cyclopeptide alkaloids were isolated from the stem bark of Ziziphus nummularia and Ziziphus spina-christi. Three previously undescribed compounds were identified: nummularine-U, spinanine-B and spinanine-C, together with the known compounds mauritine-F, nummularine-D, nummularine-E and amphibine-D. For their purification either semi-preparative HPLC with DAD and ESIMS detection or HPLC-PDA-(HRMS)-SPE-NMR was applied, together with conventional separation methods. Their structures were elucidated by spectroscopic means.
从枣椰树和酸枣的茎皮中分离出七种环肽生物碱。鉴定出三种先前未描述的化合物：枣椰碱-U、刺枣碱-B和刺枣碱-C，以及已知化合物毛里求斯碱-F、枣椰碱-D、枣椰碱-E和两栖碱-D。为了对它们进行纯化，采用了配备二极管阵列检测器（DAD）和电喷雾离子化质谱检测器（ESIMS）的半制备高效液相色谱法，或高效液相色谱-光电二极管阵列检测器-（高分辨质谱）-固相萃取-核磁共振联用技术，同时结合传统的分离方法。通过光谱手段阐明了它们的结构。
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