Wang Yi-Xin, Wang Peng, Feng Wei, Liu Chong, Yang Pan, Chen Ying-Jun, Sun Li, Sun Yang, Yue Jing, Gu Long-Jie, Zeng Qiang, Lu Wen-Qing
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
Reproductive Medicine Center, Tongji Hospital, Wuhan, Hubei, PR China.
Environ Pollut. 2017 May;224:224-234. doi: 10.1016/j.envpol.2017.01.083. Epub 2017 Mar 6.
This study aimed to investigate the relationships between environmental exposure to metals/metalloids and semen quality, sperm apoptosis and DNA integrity using the metal/metalloids levels in seminal plasma as biomarkers. We determined 18 metals/metalloids in seminal plasma using an inductively coupled plasma-mass spectrometry among 746 men recruited from a reproductive medicine center. Associations of these metals/metalloids with semen quality (n = 746), sperm apoptosis (n = 331) and DNA integrity (n = 404) were evaluated using multivariate linear and logistic regression models. After accounting for multiple comparisons and confounders, seminal plasma arsenic (As) quartiles were negatively associated with progressive and total sperm motility using multivariable linear regression analysis, which were in accordance with the trends for increased odds ratios (ORs) for below-reference semen quality parameters in the logistic models. We also found inverse correlations between cadmium (Cd) quartiles and progressive and total sperm motility, whereas positive correlations between zinc (Zn) quartiles and sperm concentration, between copper (Cu) and As quartiles and the percentage of tail DNA, between As and selenium (Se) quartiles and tail extent and tail distributed moment, and between tin (Sn) categories and the percentage of necrotic spermatozoa (all P<0.05). These relationships remained after the simultaneous consideration of various elements. Our results indicate that environmental exposure to As, Cd, Cu, Se and Sn may impair male reproductive health, whereas Zn may be beneficial to sperm concentration.
本研究旨在以精浆中金属/类金属水平作为生物标志物，调查环境暴露于金属/类金属与精液质量、精子凋亡及DNA完整性之间的关系。我们使用电感耦合等离子体质谱法测定了从一家生殖医学中心招募的746名男性精浆中的18种金属/类金属。使用多变量线性和逻辑回归模型评估了这些金属/类金属与精液质量（n = 746）、精子凋亡（n = 331）及DNA完整性（n = 404）的关联。在考虑了多重比较和混杂因素后，多变量线性回归分析显示，精浆砷（As）四分位数与精子前向运动率和总运动率呈负相关，这与逻辑模型中低于参考精液质量参数的优势比（OR）增加趋势一致。我们还发现镉（Cd）四分位数与精子前向运动率和总运动率呈负相关，而锌（Zn）四分位数与精子浓度呈正相关，铜（Cu）和As四分位数与尾部DNA百分比呈正相关，As和硒（Se）四分位数与尾部长度和尾部分布矩呈正相关，锡（Sn）类别与坏死精子百分比呈正相关（均P<0.05）。同时考虑各种元素后，这些关系依然存在。我们的结果表明，环境暴露于As、Cd、Cu、Se和Sn可能损害男性生殖健康，而Zn可能对精子浓度有益。
