Chen Gangxin, Feng YeBin, Yang Rui, Li Haiyan, Huang Zhiqin, Li Zhihao, Zheng Beihong, Sun Yan
Center of Reproductive Medicine, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Maternity and Child Health Hospital, Fujian Medical University, Fujian, China.
Fujian Maternal-Fetal Clinical Medicine Research Center, Fujian, China.
Biol Trace Elem Res. 2025 May;203(5):2561-2569. doi: 10.1007/s12011-024-04370-7. Epub 2024 Oct 3.
This study investigates the impacts of zinc ion concentration in in seminal plasma and the total amount of Zn per ejaculation on sperm quality evaluation parameters. In addition, we assessed the reliability of using zinc content in seminal plasma to evaluate sperm quality. We analyzed semen from 964 men and found that men over 40 years old had significantly lower concentrations of Zn in ejaculated semen compared to other age groups (p < 0.05), with no significant difference in Zn concentrations among other age groups (p > 0.05). The total amount of Zn in one ejaculation did not show a statistical difference between the normal semen and the abnormal semen groups (p > 0.05). Statistical differences were observed in sperm kinetic parameters and DNA fragmentation index between the normal Zn total amount group and the abnormal group (p < 0.05). However, there was no statistical difference in morphological parameters (p > 0.05). Spearman correlation analysis showed a negative correlation between Zn concentrations in seminal plasma, age, and sperm fructose and a positive correlation between semen volume, abstinence time, sperm concentration, neutral α-glycosidase, and citric acid content (p < 0.05). The receiver operating characteristic curve analysis demonstrated that Zn concentrations had poor accuracy and specificity in assessing sperm quality (p > 0.05). Although there is a partial correlation between Zn concentrations in seminal plasma and certain semen quality parameters, relying solely on Zn concentrations to evaluate sperm quality lacks accuracy and specificity.
本研究调查了精浆中锌离子浓度及每次射精锌总量对精子质量评估参数的影响。此外，我们评估了利用精浆中锌含量评估精子质量的可靠性。我们分析了964名男性的精液，发现40岁以上男性射精精液中的锌浓度显著低于其他年龄组（p<0.05），其他年龄组之间锌浓度无显著差异（p>0.05）。一次射精的锌总量在正常精液组和异常精液组之间未显示出统计学差异（p>0.05）。正常锌总量组与异常组之间在精子动力学参数和DNA碎片指数方面观察到统计学差异（p<0.05）。然而，形态学参数方面无统计学差异（p>0.05）。Spearman相关性分析显示，精浆中锌浓度、年龄与精子果糖之间呈负相关，精液量、禁欲时间、精子浓度、中性α-糖苷酶和柠檬酸含量之间呈正相关（p<0.05）。受试者工作特征曲线分析表明，锌浓度在评估精子质量方面准确性和特异性较差（p>0.05）。虽然精浆中锌浓度与某些精液质量参数之间存在部分相关性，但仅依靠锌浓度评估精子质量缺乏准确性和特异性。
