Meehan R R, Gosden J R, Rout D, Hastie N D, Friedberg T, Adesnik M, Buckland R, van Heyningen V, Fletcher J, Spurr N K
Molecular Genetics Section, Western General Hospital, Edinburgh, Scotland.
Am J Hum Genet. 1988 Jan;42(1):26-37.
The cytochrome P-450 monooxygenase system possesses catalytic activity toward many exogenous compounds (e.g., drugs, insecticides, and polycyclic aromatic hydrocarbons) and endogenous compounds (e.g., steroids, fatty acids, and prostaglandins). Multiple forms of cytochrome P-450 with different substrate specificities have been isolated. In the present paper we report the isolation and sequence of a cDNA clone for the human hepatic cytochrome P-450 responsible for mephenytoin (an anticonvulsant) oxidation. The mephenytoin cytochrome P-450 is analogous to the rat cytochrome P-450 form termed PB-1 (family P450C2C). We also report that human PB-1 is encoded by one of a small family of related genes all of which map to human chromosome 10q24.1-10q24.3. The endogenous role of this enzyme appears to be in steroid oxidations. This cytochrome P-450 family does not correspond to any of the hepatic cytochrome P-450 gene families previously mapped in humans.
细胞色素P - 450单加氧酶系统对许多外源性化合物（如药物、杀虫剂和多环芳烃）以及内源性化合物（如类固醇、脂肪酸和前列腺素）具有催化活性。已分离出多种具有不同底物特异性的细胞色素P - 450形式。在本文中，我们报告了负责美芬妥因（一种抗惊厥药）氧化的人肝细胞色素P - 450的cDNA克隆的分离和序列。美芬妥因细胞色素P - 450类似于大鼠细胞色素P - 450形式PB - 1（P450C2C家族）。我们还报告说，人PB - 1由一小族相关基因中的一个编码，所有这些基因都定位于人染色体10q24.1 - 10q24.3。这种酶的内源性作用似乎是参与类固醇氧化。这个细胞色素P - 450家族与先前在人类中定位的任何肝细胞色素P - 450基因家族都不对应。
