Babbar Anshu, Kumar Venkatesan Naveen, Bergmann René, Barrantes Israel, Pieper Dietmar H, Itzek Andreas, Nitsche-Schmitz D Patric
Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Golden Jubilee Biotech Park for Women Society, Chennai, India.
Int J Med Microbiol. 2017 Apr;307(3):174-181. doi: 10.1016/j.ijmm.2017.02.002. Epub 2017 Feb 28.
Conventionally categorized as commensals, the Streptococci of the species S. anginosus are facultative human pathogens that are difficult to diagnose and often overlooked. Furthermore, detailed investigation and diagnosis of S. anginosus infections is hampered by unexplored taxonomy and widely elusive molecular pathogenesis. To explore their pathogenic potential, S. anginosus isolates collected from patients of two geographical locations (Vellore, India and Leipzig, Germany) were subjected to multi-locus sequence analysis (MLSA). This analysis revealed the potential presence of a new distinct clade of the species S. anginosus, tentatively termed here as genomosubspecies vellorensis. A complementary PCR-based screening for S. pyogenes virulence factor as well as antibiotic resistance genes revealed not only the presence of superantigen- and extracellular DNase coding genes identical to corresponding genes of S. pyogenes, but also of erythromycin and tetracycline resistance genes in the genomes of the analyzed S. anginosus isolates, thus posing a matter of significant health concern. Identification of new pathogenic S. anginosus strains capable of causing difficult to treat infections may pose additional challenges to the diagnosis and treatment of Streptococcus based infections.
传统上被归类为共生菌的咽峡炎链球菌是兼性人类病原体，难以诊断且常被忽视。此外，咽峡炎链球菌感染的详细调查和诊断因尚未探索的分类学和广泛难以捉摸的分子发病机制而受阻。为了探索其致病潜力，对从两个地理位置（印度韦洛尔和德国莱比锡）的患者中收集的咽峡炎链球菌分离株进行了多位点序列分析（MLSA）。该分析揭示了咽峡炎链球菌可能存在一个新的独特分支，在此暂称为基因组亚种vellorensis。基于PCR的化脓性链球菌毒力因子以及抗生素抗性基因的补充筛选不仅揭示了与化脓性链球菌相应基因相同的超抗原和细胞外DNA酶编码基因的存在，还揭示了所分析的咽峡炎链球菌分离株基因组中存在红霉素和四环素抗性基因，因此引起了重大的健康问题。鉴定能够引起难以治疗的感染的新的致病性咽峡炎链球菌菌株可能会给基于链球菌的感染的诊断和治疗带来额外挑战。
