Akil Omar, Oursler A E, Fan Kevin, Lustig Lawrence R
Department of Otolaryngology-Head & Neck Surgery, University of California San Francisco, San Francisco, USA.
Bio Protoc. 2016 Mar 20;6(6). doi: 10.21769/BioProtoc.1768.
The auditory brainstem response (ABR) test provides information about the inner ear (cochlea) and the central pathways for hearing. The ABR reflects the electrical responses of both the cochlear ganglion neurons and the nuclei of the central auditory pathway to sound stimulation (Zhou , 2006; Burkard , 2007). The ABR contains 5 identifiable wave forms, labeled as I-V. Wave I represents the summated response from the spiral ganglion and auditory nerve while waves II-V represent responses from the ascending auditory pathway. The ABR is recorded via electrodes placed on the scalp of an anesthetized animal. ABR thresholds refer to the lowest sound pressure level (SPL) that can generate identifiable electrical response waves. This protocol describes the process of measuring the ABR of small rodents (mouse, rat, guinea pig, ), including anesthetizing the mouse, placing the electrodes on the scalp, recording click and tone burst stimuli and reading the obtained waveforms for ABR threshold values. As technology continues to evolve, ABR will likely provide more qualitative and quantitative information regarding the function of the auditory nerve and brainstem pathways involved in hearing.
听性脑干反应（ABR）测试可提供有关内耳（耳蜗）及听觉中枢通路的信息。ABR反映了耳蜗神经节神经元和中枢听觉通路核团对声音刺激的电反应（Zhou，2006；Burkard，2007）。ABR包含5个可识别的波形，标记为I-V。波I代表螺旋神经节和听神经的总和反应，而波II-V代表听觉上行通路的反应。ABR通过放置在麻醉动物头皮上的电极进行记录。ABR阈值是指能够产生可识别电反应波的最低声压级（SPL）。本方案描述了测量小型啮齿动物（小鼠、大鼠、豚鼠）ABR的过程，包括麻醉小鼠、将电极放置在头皮上、记录短声和短纯音刺激以及读取所获得的ABR阈值波形。随着技术不断发展，ABR可能会提供更多有关参与听觉的听神经和脑干通路功能的定性和定量信息。
