Chisi Songelwayo L, Marageni Yoanda, Naidoo Prebashni, Zulu Gloria, Akol George W, Van Heerden Henriette
Department of Agriculture and Rural Development, Allerton Provincial Veterinary Laboratory.
J S Afr Vet Assoc. 2017 Feb 28;88(0):e1-e7. doi: 10.4102/jsava.v88i0.1381.
The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples. The diagnostic specificity (DSp) of the tests mentioned above was determined using samples from known negative herds. There was no statistically significant difference between the tests in their ability to diagnose brucellosis. The RBT and iELISA had the highest DSe of 95.8%, whereas RBT and CFT had the highest DSp of 100%. In South African laboratories, the RBT and CFT serological tests are used, because of the cost efficacy of CFT when compared to the less labour intensive but more expensive iELISA.
在南非夸祖鲁 - 纳塔尔省自然感染且已知培养感染状况（金标准）的牛群中，测定了玫瑰红试验（RBT）、补体结合试验（CFT）、血清凝集试验（SAT）、竞争酶联免疫吸附测定（cELISA）和间接酶联免疫吸附测定（iELISA）的诊断敏感性（DSe）。通过从皱胃液、牛奶、滑液囊肿液、淋巴结或子宫分泌物样本中培养和鉴定流产布鲁氏菌生物变种1来确定动物的自然布鲁氏菌感染状况。使用已知阴性牛群的样本测定上述试验的诊断特异性（DSp）。这些试验在诊断布鲁氏菌病的能力上没有统计学显著差异。RBT和iELISA的DSe最高，为95.8%，而RBT和CFT的DSp最高，为100%。在南非实验室中，使用RBT和CFT血清学检测，因为与劳动强度较小但成本较高的iELISA相比，CFT具有成本效益。
