Samartino L, Gall D, Gregoret R, Nielsen K
Instituto Nacional de Tecnologia Agropecuaria-Centro de Ciencias Veterinarias y Agronómicas, Instituto de Patobiología-Bacteriología, Buenos Aires, Argentina.
Vet Microbiol. 1999 Dec;70(3-4):193-200. doi: 10.1016/s0378-1135(99)00122-4.
The purpose of this study was to evaluate the performance of the indirect enzyme immunoassay (IELISA) and the competitive enzyme immunoassay (CELISA) for the diagnosis of bovine brucellosis in comparison to conventional serological tests routinely used in Argentina. Serum samples (n = 3500), from Brucella-free herds, from vaccinated cattle and from naturally infected cattle, were tested by the following tests: buffered antigen agglutination test (BPAT), rose bengal test (RBT), 2-mercaptoethanol test (2-ME), complement fixation test (CFT), IELISA and CELISA. Sensitivity and specificity of the BPAT, RBT, IELISA and CELISA were determined relative to the 2-ME and the CFT. The CELISA was considered suitable for eliminating most serological reactions of vaccinated animals and was more specific than the other tests. The results indicate the potential use of the CELISA as a complementary assay in the brucellosis control and eradication program in Argentina and other countries, where Brucella abortusstrain 19 vaccination is mandatory.
本研究的目的是评估间接酶免疫测定法（IELISA）和竞争酶免疫测定法（CELISA）在诊断牛布鲁氏菌病方面的性能，并与阿根廷常规使用的传统血清学检测方法进行比较。对来自无布鲁氏菌牛群、接种疫苗的牛和自然感染牛的血清样本（n = 3500）进行了以下检测：缓冲抗原凝集试验（BPAT）、虎红试验（RBT）、2-巯基乙醇试验（2-ME）、补体结合试验（CFT）、IELISA和CELISA。相对于2-ME和CFT，测定了BPAT、RBT、IELISA和CELISA的敏感性和特异性。CELISA被认为适用于消除接种疫苗动物的大多数血清学反应，并且比其他检测方法更具特异性。结果表明，在阿根廷和其他强制接种流产布鲁氏菌19菌株疫苗的国家，CELISA有可能作为布鲁氏菌病控制和根除计划中的一种补充检测方法。
