用于克隆和测序EcoRI及HindIII片段的便捷载体。
Convenient vectors for cloning and sequencing EcoRI and HindIII fragments.
作者信息
Edenberg H J, Moss L G, Rutter W J
机构信息
Hormone Research Institute, University of California, San Francisco 94143-0534.
出版信息
Gene. 1987;58(2-3):297-8. doi: 10.1016/0378-1119(87)90384-2.
The polylinker regions of plasmid pUC and bacteriophage M13mp vectors have been specifically modified to provide alternative positions for cloning and reexcising EcoRI and HindIII fragments; the EcoRI and HindIII sites have been moved internal to BamHI and Bg/II sites. The location of EcoRI and HindIII sites in these HinEco vectors allows either selective linearization or excision of the cloned fragments at unique flanking sites.
质粒pUC和噬菌体M13mp载体的多克隆位点区域已进行了专门修饰，以提供用于克隆和重新切除EcoRI和HindIII片段的替代位置；EcoRI和HindIII位点已移至BamHI和Bg/II位点内部。这些HinEco载体中EcoRI和HindIII位点的位置允许在独特的侧翼位点对克隆片段进行选择性线性化或切除。
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