Crooke Almudena, Martínez-Henández Juan, Martínez-López Joaquín, Cruz-Jentoft Alfonso, Huete-Toral Fernando, Pintor Jesús
Department of Biochemistry and Molecular Biology IV, Faculty of Optics and Optometry, Universidad Complutense de Madrid, C/Arcos de Jalón 118, 28037 Madrid, Spain.
General Medical Council of Spain, Fundación para la Formación, Madrid, Spain.
Immun Ageing. 2017 May 19;14:11. doi: 10.1186/s12979-017-0094-3. eCollection 2017.
Ageing involves a progressive decline of the body's regulatory systems including immune system. Adenosine regulates immune function by interaction with its receptors, mainly adenosine A2A receptor, present on the surface of immune cells. Furthermore, cellular response to this nucleoside is highly dependent on its extracellular concentration that is regulated by ecto-enzymes such as CD39 and CD73. Therefore, the aim of this study was to investigate the effect of age on adenosine A2A receptor, CD39 and CD73 gene expression. Changes in mRNA were measured by quantitative PCR from peripheral blood of young, middle-aged and older adults as well as centenarians. Centenarians showed a prominent decrease of CD39 and CD73 mRNA in comparison with older adults. Regarding to adenosine A2A receptor, we detected two subgroups of centenarians with high and low level of transcript. Additionally, adenosine A2A receptor mRNA level of centenarians, did not correlate with their cognitive impairment. In summary, our pilot study suggests that unlike of adenosine A2A receptor, the level of CD39 as well as CD73 mRNA could be a hallmark of successful human ageing.
衰老涉及身体调节系统（包括免疫系统）的逐渐衰退。腺苷通过与免疫细胞表面的受体（主要是腺苷A2A受体）相互作用来调节免疫功能。此外，细胞对这种核苷的反应高度依赖于其细胞外浓度，而细胞外浓度由诸如CD39和CD73等外切酶调节。因此，本研究的目的是调查年龄对腺苷A2A受体、CD39和CD73基因表达的影响。通过定量PCR测量年轻人、中年人和老年人以及百岁老人外周血中mRNA的变化。与老年人相比，百岁老人的CD39和CD73 mRNA显著下降。关于腺苷A2A受体，我们检测到百岁老人中有转录水平高和低的两个亚组。此外，百岁老人的腺苷A2A受体mRNA水平与其认知障碍无关。总之，我们的初步研究表明，与腺苷A2A受体不同，CD39以及CD73 mRNA的水平可能是人类成功衰老的一个标志。
