De Clerck L S, Gigase P L, Bridts C H, Stevens W J
Section of Immunology, University of Antwerp, Belgium.
J Allergy Clin Immunol. 1988 Jan;81(1):95-9. doi: 10.1016/0091-6749(88)90226-6.
In an earlier study, we reported IgE-containing circulating immune complexes (CICs) in 66.6% of the patients with rheumatoid arthritis who were studied, especially in those with extra-articular manifestations. The present study was undertaken to examine the possible role of these immune complexes in inflammatory cell activation. Twelve patients with classic or definite rheumatoid arthritis, two with primary Sjögren's syndrome, and three patients with systemic lupus erythematosus were studied. Of these 17 patients, 10 were IgE-containing CIC positive, and seven patients were IgE-containing CIC negative. Polyethylene glycol-precipitated IgE-containing CICs and IgG-containing CICs of these patients were coated on plastic wells and incubated with suspensions of neutrophils. As a parameter of cell activation, superoxide release (SOR) was measured by cytochrome C reduction in the supernatant after 30, 60, and 90 minutes. There was a significant SOR up to 38% of the zymosan control when IgE-containing CICs were incubated with neutrophils. Furthermore, there was a significant correlation between the level of IgE-containing CICs and the amount of SOR, but not between the level of IgG-containing CICs and the amount of SOR. These results suggest a possible role for IgE-containing CICs in the activation of inflammatory cells in connective tissue diseases.
在一项早期研究中，我们报告在所研究的类风湿关节炎患者中，66.6%存在含IgE的循环免疫复合物（CIC），尤其是那些有关节外表现的患者。本研究旨在探讨这些免疫复合物在炎症细胞激活中可能发挥的作用。我们研究了12例典型或确诊的类风湿关节炎患者、2例原发性干燥综合征患者和3例系统性红斑狼疮患者。在这17例患者中，10例含IgE的CIC呈阳性，7例含IgE的CIC呈阴性。将这些患者经聚乙二醇沉淀的含IgE的CIC和含IgG的CIC包被在塑料孔板上，并与中性粒细胞悬液孵育。作为细胞激活的参数，在30、60和90分钟后，通过检测上清液中细胞色素C还原情况来测定超氧化物释放（SOR）。当含IgE的CIC与中性粒细胞孵育时，SOR显著升高，最高可达酵母聚糖对照的38%。此外，含IgE的CIC水平与SOR量之间存在显著相关性，但含IgG的CIC水平与SOR量之间不存在显著相关性。这些结果表明含IgE的CIC在结缔组织疾病的炎症细胞激活中可能发挥作用。
