Sinan Kouadio Ibrahime, Dall'Acqua Stefano, Sut Stefania, Uba Abdullahi Ibrahim, Etienne Ouattara Katinan, Ferrante Claudio, Ahmad Jamil, Zengin Gokhan
Physiology and Biochemistry Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya 42130, Turkey.
Department of Pharmacy, Botanic Garden "Giardino dei Semplici", Università degli Studi "Gabriele d'Annunzio", 66100 Chieti, Italy.
Plants (Basel). 2024 Aug 8;13(16):2195. doi: 10.3390/plants13162195.
In this work, homogenizer-assisted extraction (HAE) and maceration (MAE) were applied on leaves and bark of using water and methanol (MeOH) as solvents. HAE and MAE extracts were compared through liquid chromatography coupled with mass spectrometry (LC-MS) and evaluating the antioxidant activity, and enzyme inhibition against acetylcholinesterase (AChE), butrylcholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase. Considering the phytochemical contents and the bioassays results, the HAE extracts resulted favorably with larger content of phenolics and higher antioxidant activity. The MeOH extracts displayed the highest α-amylase inhibitory activity, with HAE MeOH leaf extract leading at 0.78 mmol acarbose equivalent (ACAE)/g. In conclusion, the study highlights that HAE can increase the extraction of phenolic and flavonoid from plant materials compared to maceration. Further research could explore the potential therapeutic applications of extracts, especially HAE MeOH leaf extracts, for their notable antioxidant and enzyme inhibitory activities, facilitating the way for the development of novel pharmaceutical interventions.
在本研究中，采用均化器辅助提取法（HAE）和浸渍法（MAE），以水和甲醇（MeOH）为溶剂，对[植物名称未给出]的叶子和树皮进行提取。通过液相色谱-质谱联用（LC-MS）对HAE和MAE提取物进行比较，并评估其抗氧化活性以及对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、酪氨酸酶、α-淀粉酶和α-葡萄糖苷酶的酶抑制作用。考虑到植物化学物质含量和生物测定结果，HAE提取物表现良好，酚类物质含量更高，抗氧化活性更强。甲醇提取物表现出最高的α-淀粉酶抑制活性，其中HAE甲醇叶提取物的抑制活性最高，为0.78 mmol阿卡波糖当量（ACAE）/g。总之，该研究强调，与浸渍法相比，HAE能够提高从[植物名称未给出]植物材料中提取酚类和黄酮类物质的效率。进一步的研究可以探索[植物名称未给出]提取物，特别是HAE甲醇叶提取物因其显著的抗氧化和酶抑制活性而具有的潜在治疗应用，为新型药物干预的开发铺平道路。
