Department of Biotechnology and Microbiology, National College (Autonomous), Tiruchirappalli, Tamil Nadu, India.
Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India.
Nat Prod Res. 2022 Jun;36(12):3216-3222. doi: 10.1080/14786419.2021.1956492. Epub 2021 Jul 24.
The present work aimed to synthesis of chitin, chitosan and sulfation of chitosan from cuttlebone of cuttlefish . Principally chitin was extracted through sequential processes of demineralisation and deproteinzation. Then chitosan was synthesized by a deacetylation and finally sulfated at semi-heterogeneous condition using chlorosulfonic acid in ,-dimethylformamide. The synthesized macromolecules were characterized for its structural, physical and thermal (CHN, DDA, FT-IR, NMR, XRD, Viscometric analysis, SEM and DSC) properties. Apart from anticoagulant potential of the sulfated chitosan was tested using human plasma by means of activated partial thromboplastin time (APTT) and prothrombin time (PT). Further sulfated chitosan was tested for antibacterial potential by well diffusion method against eleven human pathogenic clinical isolates of both Gram positive and Gram-negative strains and minimum inhibitory concentrations (MIC) was calculated accordingly. The results of this study revealed the effectiveness of the sulfated chitosan at semi-heterogeneous conditions as a potent antibacterial and anticoagulant molecule.
本工作旨在从乌贼的内壳中合成甲壳素、壳聚糖和壳聚糖的硫酸化。主要通过脱矿质和脱蛋白的顺序过程提取甲壳素。然后通过脱乙酰化合成壳聚糖，最后在半非均相条件下使用氯磺酸在二甲基甲酰胺中进行硫酸化。合成的大分子通过其结构、物理和热性质（CHN、DDA、FT-IR、NMR、XRD、粘度分析、SEM 和 DSC）进行表征。除了通过激活部分凝血活酶时间 (APTT) 和凝血酶原时间 (PT) 用人血浆测试硫酸化壳聚糖的抗凝血潜力。进一步通过扩散法测试硫酸化壳聚糖对十一株革兰氏阳性和革兰氏阴性临床分离株的抗菌潜力，并相应计算最小抑菌浓度 (MIC)。这项研究的结果表明，在半非均相条件下，硫酸化壳聚糖作为一种有效的抗菌和抗凝血分子。
