State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Metabolic Diseases Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, PR China.
Acta Biomater. 2017 May;54:128-137. doi: 10.1016/j.actbio.2017.03.011. Epub 2017 Mar 9.
Wound healing dressings are increasingly needed clinically due to the large number of skin damage annually. Nitric oxide (NO) plays a key role in promoting wound healing, thus biomaterials with NO-releasing property receive increasing attention as ideal wound dressing. In present study, we prepared a novel functional wound dressing by combining electrospun poly(ε-caprolactone) (PCL) nonwoven mat with chitosan-based NO-releasing biomaterials (CS-NO). As-prepared PCL/CS-NO dressing released NO sustainably under the physiological conditions, which was controlled by the catalysis of β-galactosidase. In vivo wound healing characteristics were further evaluated on full-thickness cutaneous wounds in mice. Results showed that PCL/CS-NO wound dressings remarkably accelerated wound healing process through enhancing re-epithelialization and granulation formation and effectively improved the organization of regenerated tissues including epidermal-dermal junction, which could be ascribed to the pro-angiogenesis, immunomodulation, and enhanced collagen synthesis provided by the sustained release of NO. Therefore, PCL/CS-NO may be a promising candidate for wound dressings, especially for the chronic wound caused by the ischemia.
Serious skin damage caused by trauma, surgery, burn or chronic disease has become one of the most serious clinical problems. Therefore, there is an increasing demand for ideal wound dressing that can improve wound healing. Due to the vital role of nitric oxide (NO), we developed a novel functional wound dressing by combining electrospun polycaprolactone (PCL) mat with NO-releasing biomaterial (CS-NO). The sustained release of NO from PCL/CS-NO demonstrated positive effects on wound healing, including pro-angiogenesis, immunomodulation, and enhanced collagen synthesis. Hence, wound healing process was remarkably accelerated and the organization of regenerated tissues was effectively improved as well. Taken together, PCL/CS-NO dressing may be a promising candidate for wound treatment, especially for the chronic wound caused by the ischemia.
由于每年大量的皮肤损伤，临床上对伤口愈合敷料的需求日益增加。一氧化氮（NO）在促进伤口愈合中起着关键作用，因此具有释放 NO 性能的生物材料作为理想的伤口敷料受到越来越多的关注。在本研究中，我们通过将静电纺聚己内酯（PCL）无纺毡与壳聚糖基 NO 释放生物材料（CS-NO）结合，制备了一种新型功能性伤口敷料。所制备的 PCL/CS-NO 敷料在生理条件下通过β-半乳糖苷酶的催化可持续释放 NO，从而控制 NO 的释放。进一步在小鼠全层皮肤损伤模型上评估了体内伤口愈合特性。结果表明，PCL/CS-NO 伤口敷料通过促进再上皮化和肉芽形成显著加速伤口愈合过程，并通过持续释放 NO 提供的促血管生成、免疫调节和增强胶原合成有效改善了再生组织的结构，包括表皮-真皮连接。因此，PCL/CS-NO 可能是一种有前途的伤口敷料候选材料，特别是对于缺血引起的慢性伤口。
