College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; University College of Agriculture, University of Sargodha, Sargodha 40100, Pakistan.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Microbiol Res. 2017 May;198:8-15. doi: 10.1016/j.micres.2017.01.008. Epub 2017 Feb 2.
Leucine-rich repeats (LRRs) domain containing kinase proteins (LRR-RK) perform various functions in eukaryotic organisms. However, their functions in Oomycetes are still largely unknown. Here, we identified an LRR-RK (PcLRR-RK1) gene and characterized its functions in Phytophthora capsici, a model oomycete specie and a major plant destroyer of solanaceous and cucurbitaceous vegetable crops. We showed that PcLRR-RK1-silenced P. capsici transformants exhibited reduced growth and produced highly branched fluffy hyphae. The shape and size of sporangia were also altered along with the reduced production of number of sporangia and zoospores. Moreover, silencing of the gene affected the cyst germination and penetration of germ tube into the host tissues, and led to the reduced virulence of P. capsici. Thus, we suggest that PcLRR-RK1 was essentially required for zoospores development, and successful infection of the P. capsici.
富含亮氨酸重复序列（LRR）结构域的激酶蛋白（LRR-RK）在真核生物中发挥着多种功能。然而，它们在卵菌中的功能在很大程度上仍然未知。在这里，我们鉴定了一个 LRR-RK（PcLRR-RK1）基因，并研究了它在辣椒疫霉中的功能。辣椒疫霉是一种模式卵菌物种，也是茄科和葫芦科蔬菜作物的主要植物破坏者。我们发现 PcLRR-RK1 沉默的辣椒疫霉转化体生长受到抑制，并产生高度分支的蓬松菌丝。此外，孢子囊的形状和大小也发生了改变，孢子囊和游动孢子的数量也减少了。而且，该基因的沉默影响了胞囊的萌发和游动孢子管侵入宿主组织的过程，导致了辣椒疫霉毒力的降低。因此，我们认为 PcLRR-RK1 对于游动孢子的发育以及辣椒疫霉的成功侵染是必不可少的。
