Si Jierui, Pei Yong, Shen Danyu, Ji Peiyun, Xu Ruofei, Xue Xue, Peng Hao, Liang Xiangxiu, Dou Daolong
Key Laboratory of Plant Immunity, College of Plant Protection, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing 210095, China.
Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6420, USA.
iScience. 2021 Jun 12;24(7):102725. doi: 10.1016/j.isci.2021.102725. eCollection 2021 Jul 23.
Leucine-rich repeat receptor-like kinases (LRR-RLKs) are critical signal receptors in plant development and defense. Like plants, oomycete pathogen genomes also harbor LRR-RLKs, but their functions remain largely unknown. Here, we systematically characterize all the 24 genes () from , which is a model of oomycete pathogens. Although none of them was required for vegetative growth, the specific PsRLKs are important for stress responses, zoospore production, zoospores chemotaxis, and pathogenicity. Interestingly, the Gα subunit PsGPA1 interacts with the five chemotaxis-related PsRLKs via their intracellular kinase domains, and expression of gene is downregulated in the three mutants (Δ). Moreover, we generated the PsRLK-PsRLK interaction network of and found that PsRLK21, together with PsRLK10 or PsRLK17, regulate virulence by direct association. Taken together, our results reveal the diverse roles of LRR-RLKs in modulating development, interaction with soybean, and responses to diverse environmental factors.
富含亮氨酸重复序列的类受体蛋白激酶（LRR-RLKs）是植物发育和防御过程中的关键信号受体。与植物一样，卵菌病原体基因组中也含有LRR-RLKs，但其功能在很大程度上仍不清楚。在这里，我们系统地表征了卵菌病原体模型——致病疫霉中的所有24个基因。虽然它们都不是营养生长所必需的，但特定的致病疫霉LRR-RLKs对胁迫反应、游动孢子产生、游动孢子趋化性和致病性很重要。有趣的是，Gα亚基PsGPA1通过其细胞内激酶结构域与五个趋化性相关的致病疫霉LRR-RLKs相互作用，并且在三个突变体（Δ）中，致病疫霉GPA1基因的表达下调。此外，我们构建了致病疫霉的LRR-RLK-LRR-RLK相互作用网络，发现PsRLK21与PsRLK10或PsRLK17一起通过直接关联调节毒力。综上所述，我们的结果揭示了LRR-RLKs在调节致病疫霉发育、与大豆的相互作用以及对多种环境因子的反应中的多种作用。
