León-Ramírez C G, Cabrera-Ponce J L, Martínez-Soto D, Sánchez-Arreguin A, Aréchiga-Carvajal E T, Ruiz-Herrera J
Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36825 Irapuato, Guanajuato, Mexico.
Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, 36825 Irapuato, Guanajuato, Mexico.
Fungal Genet Biol. 2017 Apr;101:34-45. doi: 10.1016/j.fgb.2017.02.007. Epub 2017 Mar 7.
Previously, we demonstrated that when Ustilago maydis (DC) Cda., a phytopathogenic basidiomycete and the causal agent of corn smut, is grown in the vicinity of maize embryogenic calli in a medium supplemented with the herbicide Dicamba, it developed gastroid-like basidiocarps. To elucidate the molecular mechanisms involved in the basidiocarp development by the fungus, we proceeded to analyze the transcriptome of the process, identifying a total of 2002 and 1064 differentially expressed genes at two developmental stages, young and mature basidiocarps, respectively. Function of these genes was analyzed with the use of different databases. MIPS analysis revealed that in the stage of young basidiocarp, among the ca. two thousand differentially expressed genes, there were some previously described for basidiocarp development in other fungal species. Additional elements that operated at this stage included, among others, genes encoding the transcription factors FOXO3, MIG3, PRO1, TEC1, copper and MFS transporters, and cytochromes P450. During mature basidiocarp development, important up-regulated genes included those encoding hydrophobins, laccases, and ferric reductase (FRE/NOX). The demonstration that a mapkk mutant was unable to form basidiocarps, indicated the importance of the MAPK signaling pathway in this developmental process.
此前，我们证明，当玉米黑粉病菌（Ustilago maydis (DC) Cda.），一种植物病原担子菌且是玉米瘤黑粉病的致病因子，在添加除草剂麦草畏的培养基中于玉米胚性愈伤组织附近生长时，它会发育出类腹状担子果。为阐明该真菌担子果发育所涉及的分子机制，我们着手分析这一过程的转录组，分别在两个发育阶段，即幼嫩担子果和成熟担子果阶段，共鉴定出2002个和1064个差异表达基因。利用不同数据库对这些基因的功能进行了分析。慕尼黑蛋白质序列信息中心（MIPS）分析显示，在幼嫩担子果阶段，在大约两千个差异表达基因中，有一些在其他真菌物种的担子果发育中已有描述。在此阶段起作用的其他元件包括，除其他外，编码转录因子FOXO3、MIG3、PRO1、TEC1、铜和主要易化子超家族（MFS）转运蛋白以及细胞色素P450的基因。在成熟担子果发育过程中，重要的上调基因包括那些编码疏水蛋白、漆酶和铁还原酶（FRE/NOX）的基因。一个促分裂原活化蛋白激酶（MAPK）突变体无法形成担子果的证明，表明MAPK信号通路在这一发育过程中的重要性。
