编码DNA促旋酶A亚基的大肠杆菌gyrA基因的克隆与测序。
Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase.
作者信息
Swanberg S L, Wang J C
机构信息
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
出版信息
J Mol Biol. 1987 Oct 20;197(4):729-36. doi: 10.1016/0022-2836(87)90479-7.
The gene gyrA of Escherichia coli, which encodes the A subunit of DNA gyrase (topoisomerase II), has been cloned and a region of approximately 3300 base-pairs sequenced. An open reading frame of 2625 nucleotides coding for a protein of 97,000 Mr is located. The peptide weight of the subunit predicted from this open reading frame is in close agreement with previously published estimates of that of the A subunit. There is a "TATAAT" promoter motif located 44 bases upstream from the first "ATG" of the open reading frame. The amino acid sequence derived from the nucleotide sequence is about 50% homologous with that derived from the Bacillus subtilis gyrA gene sequence, with several regions showing greater than 90% homology.
编码DNA促旋酶（拓扑异构酶II）A亚基的大肠杆菌基因gyrA已被克隆，并对大约3300个碱基对的区域进行了测序。发现了一个2625个核苷酸的开放阅读框，其编码一种97,000道尔顿的蛋白质。从这个开放阅读框预测的亚基肽重量与先前发表的A亚基估计值非常一致。在开放阅读框第一个“ATG”上游44个碱基处有一个“TATAAT”启动子基序。从核苷酸序列推导的氨基酸序列与从枯草芽孢杆菌gyrA基因序列推导的氨基酸序列约有50%的同源性，有几个区域显示出大于90%的同源性。
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