Rainieri Sandra, Conlledo Nadia, Langerholc Tomaž, Madorran Eneko, Sala Martin, Barranco Alejandro
Food Research Division, AZTI, Parque Tecnológico de Bizkaia, Astondo Bidea 609, 48160 Derio, Spain.
Food Research Division, AZTI, Parque Tecnológico de Bizkaia, Astondo Bidea 609, 48160 Derio, Spain.
Food Chem Toxicol. 2017 Jun;104:14-25. doi: 10.1016/j.fct.2017.02.041. Epub 2017 Mar 9.
This work aims at deepening the understanding of the mode of action of some of the most prominent perfluorinated compounds (PFCs) by detecting in a realistic way their effects. To this end, after adjusting the exposure media taking into account the biological model employed and the physico-chemical properties of PFCs, we evaluated the toxic effects of PFOA, PFOS and PFNA in a human macrophage cell line (TLT cells) and in zebrafish embryos. We performed such evaluation on individual compounds and mixtures. Acute toxicity was greater for PFOS in zebrafish; however, it was greater for PFNA in TLT cells. PFNA was also the compound producing the greatest levels of oxidative stress, both in zebrafish and TLT cells. Additionally, in both biological systems, it showed a much stronger effect on mixtures in comparison to the others PFCs tested in this work. Mixture studies in zebrafish showed that acute toxicity depended on the concentration and that the mixture was far more toxic than the individual compounds. This study highlights the importance of studying PFCs in realistic conditions on various biological models.
这项工作旨在通过实际检测某些最突出的全氟化合物（PFCs）的作用方式，加深对其的理解。为此，在考虑所采用的生物学模型和PFCs的物理化学性质后对暴露介质进行调整，我们评估了全氟辛酸（PFOA）、全氟辛烷磺酸（PFOS）和全氟萘酸（PFNA）对人巨噬细胞系（TLT细胞）和斑马鱼胚胎的毒性作用。我们对单一化合物和混合物进行了此类评估。PFOS对斑马鱼的急性毒性更大；然而，PFNA对TLT细胞的急性毒性更大。PFNA也是在斑马鱼和TLT细胞中产生最高水平氧化应激的化合物。此外，在这两种生物系统中，与本研究中测试的其他PFCs相比，它对混合物的影响要强得多。斑马鱼的混合物研究表明，急性毒性取决于浓度，并且混合物的毒性远高于单一化合物。这项研究突出了在实际条件下对各种生物模型进行PFCs研究的重要性。
