Souza Thiara Manuele Alves, Azeredo Elzinandes Leal, Badolato-Corrêa Jessica, Damasco Paulo Vieira, Santos Carla, Petitinga-Paiva Fabienne, Nunes Priscila Conrado Guerra, Barbosa Luciana Santos, Cipitelli Márcio Costa, Chouin-Carneiro Thais, Faria Nieli Rodrigues Costa, Nogueira Rita Maria Ribeiro, de Bruycker-Nogueira Fernanda, Dos Santos Flavia Barreto
Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.
Rio-Laranjeiras Hospital, Gaffrée Guinle University Hospital (Federal University of the State of Rio de Janeiro), Rio de Janeiro, Brazil Pedro Ernesto University Hospital (University of the State of Rio de Janeiro), Rio de Janeiro, Brazil.
PLoS Curr. 2017 Feb 14;9:ecurrents.outbreaks.4200119978d62ccaa454599cd2735727. doi: 10.1371/currents.outbreaks.4200119978d62ccaa454599cd2735727.
Chikungunya virus (CHIKV) is an arbovirus that causes an acute febrile syndrome with a severe and debilitating arthralgia. In Brazil, the Asian and East-Central South African (ECSA) genotypes are circulating in the north and northeast of the country, respectively. In 2015, the first autochthonous cases in Rio de Janeiro, Brazil were reported but until now the circulating strains have not been characterized. Therefore, we aimed here to perform the molecular characterization and phylogenetic analysis of CHIKV strains circulating in the 2016 outbreak occurred in the municipality of Rio de Janeiro.
The cases analyzed in this study were collected at a private Hospital, from April 2016 to May 2016, during the chikungunya outbreak in Rio de Janeiro, Brazil. All cases were submitted to the Real Time RT-PCR for CHIKV genome detection and to anti-CHIKV IgM ELISA. Chikungunya infection was laboratorially confirmed by at least one diagnostic method and, randomly selected positive cases (n=10), were partially sequenced (CHIKV E1 gene) and analyzed.
The results showed that all the samples grouped in ECSA genotype branch and the molecular characterization of the fragment did not reveal the A226V mutation in the Rio de Janeiro strains analyzed, but a K211T amino acid substitution was observed for the first time in all samples and a V156A substitution in two of ten samples.
Phylogenetic analysis and molecular characterization reveals the circulation of the ECSA genotype of CHIKV in the city of Rio de Janeiro, Brazil and two amino acids substitutions (K211T and V156A) exclusive to the CHIKV strains obtained during the 2016 epidemic, were reported.
基孔肯雅病毒（CHIKV）是一种虫媒病毒，可引起伴有严重且使人衰弱的关节痛的急性发热综合征。在巴西，亚洲基因型和东中非南非（ECSA）基因型分别在该国北部和东北部传播。2015年，巴西里约热内卢报告了首例本土病例，但截至目前，所传播的毒株尚未得到鉴定。因此，我们旨在对2016年里约热内卢市疫情中传播的CHIKV毒株进行分子鉴定和系统发育分析。
本研究分析的病例于2016年4月至2016年5月在巴西里约热内卢基孔肯雅热疫情期间从一家私立医院收集。所有病例均接受了用于CHIKV基因组检测的实时逆转录聚合酶链反应（Real Time RT-PCR）以及抗CHIKV IgM酶联免疫吸附测定（ELISA）。通过至少一种诊断方法在实验室确诊基孔肯雅热感染，并对随机选择的阳性病例（n = 10）进行部分测序（CHIKV E1基因）并分析。
结果显示，所有样本均归入ECSA基因型分支，对所分析的里约热内卢毒株片段的分子鉴定未发现A226V突变，但在所有样本中首次观察到K211T氨基酸取代，在十个样本中的两个样本中观察到V156A取代。
系统发育分析和分子鉴定揭示了巴西里约热内卢市CHIKV的ECSA基因型的传播情况，并报告了2016年疫情期间获得的CHIKV毒株特有的两个氨基酸取代（K211T和V156A）。
