Ray R, Glaze B J, Compans R W
Molecular Engineering Associates, Inc., Birmingham, Alabama 35233.
J Virol. 1988 Mar;62(3):783-7. doi: 10.1128/JVI.62.3.783-787.1988.
Affinity-purified hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of human parainfluenza virus type 3 (P13 virus) were used to investigate their role in the induction of a protective immune response following immunization of hamsters. The efficacy of immunization with the glycoprotein antigens was tested by challenge infection. Results of virus recovery from lungs and trachea demonstrated that although immunization with HN or F alone induced an antibody response to the respective glycoproteins, it did not provide a significant level of protection. However, immunization with a mixture of both purified glycoproteins induced higher virus-neutralizing activity in bronchial lavages and afforded complete protection from challenge infection. Similarly, incomplete protection was observed after passive transfer of monospecific rabbit antibody to the purified HN or F in baby hamsters. On the other hand, passive transfer of a mixture of antibodies to HN and F conferred a higher level of protection. Thus, the presence of antibody to both glycoproteins of P13 virus may be essential for protective immunity.
利用亲和纯化的3型人副流感病毒（P13病毒）血凝素神经氨酸酶（HN）和融合（F）糖蛋白，研究它们在仓鼠免疫后诱导保护性免疫反应中的作用。通过攻毒感染来测试糖蛋白抗原免疫的效果。从肺和气管中回收病毒的结果表明，虽然单独用HN或F免疫可诱导针对相应糖蛋白的抗体反应，但并未提供显著水平的保护。然而，用两种纯化糖蛋白的混合物免疫可在支气管灌洗中诱导更高的病毒中和活性，并提供完全的攻毒感染保护。同样，在幼仓鼠中被动转移针对纯化HN或F的单特异性兔抗体后，观察到不完全保护。另一方面，将针对HN和F的抗体混合物被动转移可提供更高水平的保护。因此，针对P13病毒两种糖蛋白的抗体的存在可能是保护性免疫所必需的。
