Ray R, Matsuoka Y, Burnett T L, Glaze B J, Compans R W
Molecular Engineering Associates, Birmingham, Alabama 35205.
J Infect Dis. 1990 Sep;162(3):746-9. doi: 10.1093/infdis/162.3.746.
Induction of type-specific and cross-protective immune responses against human parainfluenza viruses have been investigated. The envelope glycoproteins HN (76 kDa) and F0 (62 kDa) from parainfluenza type 2 virus were selectively solubilized with octylglucoside. Detergent-soluble envelope glycoproteins were used as vaccine antigens for intranasal immunization of hamsters. The immunized animals showed complete protection from challenge infection with prototype live virus but failed to demonstrate a significant level of protection against either human parainfluenza type 1 or type 3 virus. The sera and bronchial lavages of immunized animals also showed type-specific neutralizing antibodies. A similar type-specific protective immune response was also noted after primary infection with live virus. The results indicate that a multivalent parainfluenza virus vaccine is probably required for protection against natural infection.
针对人副流感病毒的型特异性和交叉保护性免疫反应的诱导已得到研究。用辛基葡糖苷选择性地溶解来自2型副流感病毒的包膜糖蛋白HN（76 kDa）和F0（62 kDa）。去污剂可溶的包膜糖蛋白用作仓鼠鼻内免疫的疫苗抗原。免疫的动物对原型活病毒的攻击感染显示出完全保护，但对人1型或3型副流感病毒未能显示出显著水平的保护。免疫动物的血清和支气管灌洗也显示出型特异性中和抗体。初次感染活病毒后也观察到类似的型特异性保护性免疫反应。结果表明，可能需要一种多价副流感病毒疫苗来预防自然感染。
