Slobodkina G B, Baslerov R V, Novikov A A, Bonch-Osmolovskaya E A, Slobodkin A I
Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russian Federation.
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russian Federation.
Int J Syst Evol Microbiol. 2017 Feb;67(2):301-305. doi: 10.1099/ijsem.0.001619.
A novel thermophilic, anaerobic, chemolithoautotrophic bacterium, strain SF97T, was isolated from a terrestrial hot spring (Kuril Islands, Russia). Cells of strain SF97T were rod-shaped and motile with a Gram-positive cell-wall type. The novel isolate grew at 45-72 °C (optimum 65 °C) and pH 5.5-8.5 (optimum 6.0-6.5). The strain grew chemolithoautotrophically with molecular hydrogen as an electron donor, sodium sulfite or SO2 gas as an electron acceptor and bicarbonate/CO2 as a carbon source. Sulfate, thiosulfate, elemental sulfur, Fe(III) or nitrate were not used as electron acceptors either with H2 or organic electron donors. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the family Thermoanaerobacteraceae, order Thermoanaerobacterales, and was distantly related to species of the genus Ammonifex (92-93 % sequence similarity). On the basis of its physiological properties and results of phylogenetic analyses, strain SF97T is considered to represent a novel species of a new genus, for which the name Thermodesulfitimonas autotrophica gen. nov., sp. nov. is proposed. The type strain of Thermodesulfitimonas autotrophica is SF97T (=DSM 102936T=VKM B-2961T). T. autotrophica is the first reported obligate sulfite-reducing micro-organism.
从陆地温泉（俄罗斯千岛群岛）中分离出一株新型嗜热、厌氧、化能无机自养细菌，菌株SF97T。菌株SF97T的细胞呈杆状，具运动性，细胞壁类型为革兰氏阳性。该新型分离株在45-72 °C（最适温度65 °C）和pH 5.5-8.5（最适pH 6.0-6.5）条件下生长。该菌株以分子氢作为电子供体、亚硫酸钠或二氧化硫气体作为电子受体、碳酸氢盐/二氧化碳作为碳源进行化能无机自养生长。无论是与氢气还是有机电子供体一起，硫酸盐、硫代硫酸盐、元素硫、Fe(III)或硝酸盐都不被用作电子受体。基于16S rRNA基因序列的系统发育分析表明，该分离株属于嗜热厌氧杆菌科、嗜热厌氧杆菌目，与产氨杆菌属的物种亲缘关系较远（序列相似性为92-93 %）。根据其生理特性和系统发育分析结果，菌株SF97T被认为代表一个新属的一个新物种，为此提出新属名嗜热亚硫酸盐还原单胞菌属（Thermodesulfitimonas）、新种名自养嗜热亚硫酸盐还原单胞菌（Thermodesulfitimonas autotrophica）。自养嗜热亚硫酸盐还原单胞菌的模式菌株为SF97T（=DSM 102936T=VKM B-2961T）。自养嗜热亚硫酸盐还原单胞菌是首个被报道的专性亚硫酸盐还原微生物。
