Avanesyan Alina, Jaffe Benjamin D, Guédot Christelle
Department of Entomology, University of Wisconsin-Madison, 1630 Linden Drive Madison, WI 53706, USA.
Insects. 2017 Mar 10;8(1):32. doi: 10.3390/insects8010032.
The spotted wing drosophila, (Diptera: Drosophilidae), is an emerging invasive pest, which attacks a wide variety of fruits and berries. Although previous studies have focused on different aspects of reproductive biology, there are no protocols available for determining the mating status of females and drosophilids in general. In this study, a step-by-step protocol for tissue dissection, isolating spermathecae, and determining the mating status of females was developed specifically for . This protocol is an effective and relatively quick method for determining female mating status. It has important applications from exploring reproductive output of females to understanding the biology of winter morph, which presumably plays the main role in the overwintering of this invasive species. We demonstrated applicability of this protocol for both field collected flies and flies reared in the lab, including fly specimens stored on a long-term basis.
斑翅果蝇（双翅目：果蝇科）是一种新出现的入侵性害虫，它会攻击多种水果和浆果。尽管先前的研究集中在生殖生物学的不同方面，但目前尚无用于确定雌性果蝇及一般果蝇交配状态的方案。在本研究中，专门为斑翅果蝇制定了一套用于组织解剖、分离受精囊以及确定雌性交配状态的分步方案。该方案是确定雌性交配状态的一种有效且相对快速的方法。它在从探究雌性生殖产出到理解冬季形态的生物学特性等方面都有重要应用，而冬季形态可能在这种入侵物种的越冬过程中起主要作用。我们证明了该方案对野外采集的果蝇以及实验室饲养的果蝇均适用，包括长期保存的果蝇标本。
