Salinero Juan J, Lara Beatriz, Ruiz-Vicente Diana, Areces Francisco, Puente-Torres Carlos, Gallo-Salazar César, Pascual Teodoro, Del Coso Juan
Exercise Physiology Laboratory, Camilo José Cela University, Madrid 28692, Spain.
Nutrients. 2017 Mar 11;9(3):269. doi: 10.3390/nu9030269.
Previous investigations have determined that some individuals have minimal or even ergolytic performance effects after caffeine ingestion. The aim of this study was to analyze the influence of the genetic variations of the CYP1A2 gene on the performance enhancement effects of ingesting a moderate dose of caffeine. In a double-blind randomized experimental design, 21 healthy active participants (29.3 ± 7.7 years) ingested 3 mg of caffeine per kg of body mass or a placebo in testing sessions separated by one week. Performance in the 30 s Wingate test, visual attention, and side effects were evaluated. DNA was obtained from whole blood samples and the CYP1A2 polymorphism was analyzed (rs762551). We obtained two groups: AA homozygotes ( = 5) and C-allele carriers ( = 16). Caffeine ingestion increased peak power (682 ± 140 vs. 667 ± 137 W; = 0.008) and mean power during the Wingate test (527 ± 111 vs. 518 ± 111 W; < 0.001) with no differences between AA homozygotes and C-allele carriers ( > 0.05). Reaction times were similar between caffeine and placebo conditions (276 ± 31 vs. 269 ± 71 milliseconds; = 0.681) with no differences between AA homozygotes and C-allele carriers. However, 31.3% of the C-allele carriers reported increased nervousness after caffeine ingestion, while none of the AA homozygotes perceived this side effect. Genetic variations of the CYP1A2 polymorphism did not affect the ergogenic effects and drawbacks derived from the ingestion of a moderate dose of caffeine.
先前的研究已确定，一些人在摄入咖啡因后对运动能力的影响很小甚至产生运动能力下降的影响。本研究的目的是分析CYP1A2基因的遗传变异对摄入中等剂量咖啡因后运动能力增强效果的影响。在双盲随机实验设计中，21名健康的活跃参与者（29.3±7.7岁）在间隔一周的测试环节中，每千克体重摄入3毫克咖啡因或安慰剂。评估了30秒温盖特测试中的运动表现、视觉注意力和副作用。从全血样本中获取DNA，并分析CYP1A2基因多态性（rs762551）。我们得到了两组：AA纯合子（n = 5）和C等位基因携带者（n = 16）。摄入咖啡因后，温盖特测试中的峰值功率增加（682±140 vs. 667±137瓦；P = 0.008），平均功率增加（527±111 vs. 518±111瓦；P < 0.001），AA纯合子和C等位基因携带者之间无差异（P > 0.05）。咖啡因和安慰剂条件下的反应时间相似（276±31 vs. 269±71毫秒；P = 0.681），AA纯合子和C等位基因携带者之间无差异。然而，31.3%的C等位基因携带者报告摄入咖啡因后紧张感增加，而AA纯合子均未察觉到这种副作用。CYP1A2基因多态性的遗传变异并未影响摄入中等剂量咖啡因所产生的运动能力增强效果和不良影响。
