Sun Xi-Feng, Zhao Li, Zhang Zhen-Tang, Liu Miao-Miao, Xue Zai-Feng, Wen Hong-Ling, Ma Dong-Qiang, Huang Yu-Ting, Sun Yue, Zhou Chuan-Min, Luo Li-Mei, Liu Jian-Wei, Li Wen-Qian, Yu Hao, Yu Xue-Jie
1 School of Public Health, Shandong University , Jinan, China .
2 Huangdao District Center for Disease Control and Prevention , Qingdao City, China .
Vector Borne Zoonotic Dis. 2017 Jun;17(6):425-431. doi: 10.1089/vbz.2016.2056. Epub 2017 Mar 13.
Recently, hantaviruses have been discovered in insectivores in Europe, Asia, Africa, and North America. Imjin virus (MJNV) was first isolated from the lung tissues of Ussuri white-toothed shrew (Crocidura lasiura) from South Korea in 2009. We aim to detect the species and prevalence of insectivore- and rodent-borne hantaviruses in shrews and rodents.
Shrews and rodents were captured in Jiaonan County of Shandong Province, China, in 2014. RT-PCR was used to amplify viral RNA of Hantavirus species, including insectivore-borne Imjin virus (MJNV), rodent-borne Hantaan virus (HTNV), and Seoul virus (SEOV) from shrews and rodents.
We found that MJNV infected 10.7% (19/178) of Crocidura shrews, but it infected none of rodents (0/475); we also found that 2 of 178 (1.1%) Crocidura shrews were PCR positive to SEOV. This study indicated that the major animal hosts of Imjin virus are shrews, and rodent-borne SEOV can infect shrews.
最近，在欧洲、亚洲、非洲和北美洲的食虫动物中发现了汉坦病毒。2009年，从韩国的乌苏里白齿鼩（Crocidura lasiura）的肺组织中首次分离出临津江病毒（MJNV）。我们旨在检测食虫动物和啮齿动物携带的汉坦病毒在鼩鼱和啮齿动物中的种类及流行情况。
2014年在中国山东省胶南县捕获鼩鼱和啮齿动物。采用逆转录聚合酶链反应（RT-PCR）从鼩鼱和啮齿动物中扩增汉坦病毒属的病毒RNA，包括食虫动物携带的临津江病毒（MJNV）、啮齿动物携带的汉滩病毒（HTNV）和汉城病毒（SEOV）。
我们发现临津江病毒感染了10.7%（19/178）的臭鼩鼱，但未感染任何啮齿动物（0/475）；我们还发现178只臭鼩鼱中有2只（1.1%）对汉城病毒PCR检测呈阳性。本研究表明临津江病毒的主要动物宿主是鼩鼱，且啮齿动物携带的汉城病毒可感染鼩鼱。
