在中国，亚洲鼩鼱（Suncus murinus）携带的一种新的托塔帕莱姆病毒亚型。
A new subtype of Thottapalayam virus carried by the Asian house shrew (Suncus murinus) in China.
机构信息
State Key Laboratory for Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, China.
出版信息
Infect Genet Evol. 2011 Dec;11(8):1862-7. doi: 10.1016/j.meegid.2011.07.013. Epub 2011 Jul 27.
Recently, many novel hantavirus species carried by insectivore had been found. In order to know whether the insectivore-associated hantaviruses exist in China, Asian house shrew (Suncus murinus) were analyzed for the presence of hantaviruses. 362 Asian house shrews were captured in Wenzhou of Zhejiang province. Hantavirus-specific RNA was detected by RT-PCR in lung tissue samples of six shrews. The complete hantavirus S and M segment sequences and also partial L segment sequences were successfully recovered. Pairwise comparison of the nucleotide and deduced amino acid sequences showed that the newly discovered virus strains are most closely related to Thottapalayam virus (TPMV) isolated from S. murinus in India, with 82.5-84.7% sequence identity at the nucleotide level and 95.0-98.2% at the amino acid level. Corresponding sequences of Imjin virus (MJNV) were more distant and other rodent- and insectivore-associated hantaviruses appeared even less related. Phylogenetic analysis revealed that the new variants formed a distinct lineage within TPMV.
最近，发现了许多由食虫动物携带的新型汉坦病毒。为了了解中国是否存在食虫动物相关的汉坦病毒，对亚洲鼩鼱（Suncus murinus）进行了分析。在浙江省温州市捕获了 362 只亚洲鼩鼱。通过 RT-PCR 在六只鼩鼱的肺组织样本中检测到汉坦病毒特异性 RNA。成功回收了完整的汉坦病毒 S 和 M 片段序列以及部分 L 片段序列。核苷酸和推导的氨基酸序列的成对比较表明，新发现的病毒株与从印度亚洲鼩鼱中分离的 Thottapalayam 病毒（TPMV）最为密切相关，核苷酸水平的序列同一性为 82.5-84.7%，氨基酸水平的序列同一性为 95.0-98.2%。与 Imjin 病毒（MJNV）的相应序列则更为遥远，其他与啮齿动物和食虫动物相关的汉坦病毒则显得更为不相关。系统发育分析表明，新变体在 TPMV 内形成了一个独特的谱系。
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