Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Department of Biological Sciences, National University of Singapore, Singapore.
BMB Rep. 2017 May;50(5):247-256. doi: 10.5483/bmbrep.2017.50.5.044.
CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research. [BMB Reports 2017; 50(5): 247-256].
CRISPR/Cas9 是基因组工程领域引入的最新工具，与先前的工具相比，如 Meganucleases、锌指核酸酶（ZFNs）和转录激活因子样效应物（TALENs），它是迄今为止最好的基因组编辑工具。CRISPR/Cas9 系统的简单设计和组装使得基因组编辑变得容易，因为它使用与 DNA 靶标相对应的小向导 RNA 进行高效编辑。这有助于为许多使用旧遗传干扰技术难以处理的物种打开多重基因组靶向的大门。目前，CRISPR 系统正在彻底改变生物研究的方式，不仅为研究，而且为医学和生物技术铺平了光明的未来。在这篇综述中，我们评估了 CRISPR/Cas 系统的历史、类型和结构、作用机制。特别关注了这个强大工具在自噬研究中的应用。[BMB 报告 2017;50(5):247-256]。
