Umair Saleh, Pernthaner Anton, Deng Qing, Gibson Blake, Hook Sarah, Heath David
The Hopkirk Research Institute, AgResearch Ltd, Private Bag 11-008, Palmerston North, New Zealand.
The Hopkirk Research Institute, AgResearch Ltd, Private Bag 11-008, Palmerston North, New Zealand.
Vet Parasitol. 2017 Mar 15;236:117-120. doi: 10.1016/j.vetpar.2017.02.007. Epub 2017 Feb 14.
The EG95 vaccine is effective in protecting grazing animals from infection with Echinococcus granulosus. Six male lambs were used in the study, two were each vaccinated subcutaneously with 50μg EG95/1mg Quil-A, two animals were each vaccinated with 50μg EG95/1mg Quil-A in 1% chitosan thermolabile gel subcutaneously, and two animals served as non-vaccinated controls. Two vaccinations were given at a 7 week interval. Two vaccinations induced a significantly higher antibody titre in the chitosan group compared with the Quil-A only group. The chitosan vaccine group also had a significantly higher antibody titre compared with a positive control sera from vaccinated and challenged sheep. Incorporating the EG95/Quil-A vaccine in a thermo-responsive chitosan sol-gel stimulated, after the second injection, a high level of antibody absorbance which remained high for at least one year. This response was significantly greater than the response to vaccine without the gel.
EG95疫苗可有效保护放牧动物免受细粒棘球绦虫感染。该研究使用了6只雄性羔羊，其中2只每只皮下注射50μg EG95/1mg Quil-A进行疫苗接种，2只每只皮下注射含50μg EG95/1mg Quil-A的1%壳聚糖热不稳定凝胶进行疫苗接种，另外2只作为未接种疫苗的对照。每隔7周进行两次疫苗接种。与仅使用Quil-A的组相比，壳聚糖组两次疫苗接种诱导产生的抗体滴度显著更高。壳聚糖疫苗组的抗体滴度也显著高于来自接种并攻毒绵羊的阳性对照血清。在第二次注射后，将EG95/Quil-A疫苗掺入热响应性壳聚糖溶胶-凝胶中可刺激产生高水平的抗体吸光度，且该水平至少一年内保持较高。这种反应明显大于对不含凝胶的疫苗的反应。
