Wang Q P, Kaguni J M
Department of Biochemistry, Michigan State University, East Lansing 48824.
Mol Gen Genet. 1987 Oct;209(3):518-25. doi: 10.1007/BF00331158.
The promoter region of the dnaA gene and of a gene which encodes a 16 kDa protein contain sites which are recognized and bound by dnaA protein. Using assays of run-off transcription of restriction fragments, purified dnaA protein specifically repressed transcription from both dnaA promoters and from the promoter for the 16 KD gene to almost undetectable levels. This repressive effect was observed at levels of dnaA protein required for specific binding of dnaA protein to restriction fragments containing the promoters for these genes. These results indicate that transcription of these genes is regulated by binding of dnaA protein to the promoter regions of these genes.
dnaA基因以及编码一种16kDa蛋白质的基因的启动子区域含有可被dnaA蛋白识别并结合的位点。通过对限制片段进行连续转录分析，纯化的dnaA蛋白可特异性地将来自dnaA两个启动子以及16KD基因启动子的转录抑制到几乎检测不到的水平。在dnaA蛋白与含有这些基因启动子的限制片段特异性结合所需的dnaA蛋白水平上观察到了这种抑制作用。这些结果表明，这些基因的转录是通过dnaA蛋白与这些基因启动子区域的结合来调控的。
