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一个人类甲硫氨酸起始转运RNA（tRNA<sup>(iMet)</sup>）基因产生多种转录本。

A human tRNA(iMet) gene produces multiple transcripts.

作者信息

Vnencak-Jones C L, Wahab S Z, Zehner Z E, Holmes W M

机构信息

Department of Human Genetics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.

出版信息

Mol Cell Biol. 1987 Nov;7(11):4134-8. doi: 10.1128/mcb.7.11.4134-4138.1987.

DOI:10.1128/mcb.7.11.4134-4138.1987
PMID:2828928
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368087/
Abstract

A third nonallelic locus of the human methionyl-tRNA multigene family (tRNA(iMet-3) was isolated. This gene, unlike two other tRNA(iMet) loci, lacks a remarkable run of T and C residues which functions as a termination of transcription signal. Instead, three tandem termination signals, each containing no more than four thymidylate residues, function as relatively inefficient termination signals. As a result, polymerase readthrough generates at least three transcripts in vitro. The efficiency of apparent termination varies significantly at these sites. All resulting transcripts appear to be processed in vitro.

摘要

分离出了人类甲硫氨酰 - tRNA多基因家族的第三个非等位基因座（tRNA(iMet-3)）。与其他两个tRNA(iMet)基因座不同，该基因缺乏一串显著的T和C残基，而这串残基作为转录终止信号发挥作用。取而代之的是三个串联的终止信号，每个信号包含不超过四个胸苷酸残基，作为相对低效的终止信号。因此，聚合酶通读在体外产生至少三种转录本。在这些位点，明显的终止效率差异很大。所有产生的转录本在体外似乎都经过了加工。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239d/368087/1d0e02af06a3/molcellb00083-0283-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239d/368087/53ee692c126f/molcellb00083-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239d/368087/9a3d8e6dbd07/molcellb00083-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239d/368087/1d0e02af06a3/molcellb00083-0283-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239d/368087/53ee692c126f/molcellb00083-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239d/368087/9a3d8e6dbd07/molcellb00083-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239d/368087/1d0e02af06a3/molcellb00083-0283-b.jpg

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本文引用的文献

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Proc Natl Acad Sci U S A. 1983 Jun;80(11):3416-20. doi: 10.1073/pnas.80.11.3416.
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Silkworm 5S RNA and alanine tRNA genes share highly conserved 5' flanking and coding sequences.家蚕5S核糖体RNA和丙氨酸转运RNA基因具有高度保守的5'侧翼序列和编码序列。
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Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
真核生物RNA聚合酶III介导的转录终止
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Nucleic Acids Res. 2011 Jul;39(13):5499-512. doi: 10.1093/nar/gkr074. Epub 2011 Mar 17.
RNA聚合酶III对纯化基因进行准确转录需要多种因素。
J Biol Chem. 1980 Dec 25;255(24):11986-91.
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Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.真核生物tRNA基因中的两个保守序列块是主要的启动子元件。
Nature. 1981 Dec 17;294(5842):626-31. doi: 10.1038/294626a0.
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A split promoter for a eucaryotic tRNA gene.一种真核生物tRNA基因的分裂启动子。
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