Smirnova T A, Bogdanova T L, Gal'perin M Iu, Grigor'eva T M, Azizbekian R R
Mol Gen Mikrobiol Virusol. 1987 Oct(10):23-7.
The possibility of homologous and heterologous transception of Cry+ plasmids in Bacillus thuringiensis is demonstrated. Cry+ plasmids from crystal bearing strain of Bacillus thuringiensis were transferred into acrystalline strain belonging to H5 serotype by mutual incubation. The donor strain was previously marked by the transmissive plasmid pAM beta 1 coding for erythromycin and lincomycin resistance. The transcipients having acquired the ability to synthesize delta-endotoxin were referred to H5 serotype due to their phenotype. By analogous method Cry+ plasmid was transferred from Bacillus thuringiensis to Bacillus cereus. Bacillus cereus strain GP7 was used as a recipient strain resistant to tetracycline. The presence of delta-endotoxin in transcipients was confirmed by bioprobes and immunoenzyme assay. To prove the transfer of Cry+ plasmid the plasmid profiles of the parent strains and transcipients have been analyzed. The formation of cellular contacts during mutual incubation of Bacillus thuringiensis and Bacillus cereus strains was demonstrated by electron microscopic study of ultrafine cuts.
苏云金芽孢杆菌中Cry+质粒同源和异源转导的可能性得到了证实。通过共同培养，将来自苏云金芽孢杆菌晶体携带菌株的Cry+质粒转移到属于H5血清型的无晶体菌株中。供体菌株先前用编码红霉素和林可霉素抗性的可转移质粒pAMβ1进行了标记。获得合成δ-内毒素能力的转导子因其表型而被归为H5血清型。通过类似方法，Cry+质粒从苏云金芽孢杆菌转移到蜡样芽孢杆菌。蜡样芽孢杆菌菌株GP7用作对四环素耐药的受体菌株。通过生物探针和免疫酶测定法证实了转导子中存在δ-内毒素。为了证明Cry+质粒的转移，分析了亲本菌株和转导子的质粒图谱。通过对超薄切片的电子显微镜研究，证实了苏云金芽孢杆菌和蜡样芽孢杆菌菌株共同培养期间细胞接触的形成。
