Zhang Xinye, Valdés-López Oswaldo, Arellano Consuelo, Stacey Gary, Balint-Kurti Peter
Maize Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, 611130, Sichuan, China.
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA.
Theor Appl Genet. 2017 Jun;130(6):1155-1168. doi: 10.1007/s00122-017-2876-6. Epub 2017 Mar 13.
Loci associated with variation in maize responses to two microbe-associated molecular patterns (MAMPs) were identified. MAMP responses were correlated. No relationship between MAMP responses and quantitative disease resistance was identified. Microbe-associated molecular patterns (MAMPs) are highly conserved molecules commonly found in microbes which can be recognized by plant pattern recognition receptors. Recognition triggers a suite of responses including production of reactive oxygen species (ROS) and nitric oxide (NO) and expression changes of defense-related genes. In this study, we used two well-studied MAMPs (flg22 and chitooctaose) to challenge different maize lines to determine whether there was variation in the level of responses to these MAMPs, to dissect the genetic basis underlying that variation and to understand the relationship between MAMP response and quantitative disease resistance (QDR). Naturally occurring quantitative variation in ROS, NO production, and defense genes expression levels triggered by MAMPs was observed. A major quantitative traits locus (QTL) associated with variation in the ROS production response to both flg22 and chitooctaose was identified on chromosome 2 in a recombinant inbred line (RIL) population derived from the maize inbred lines B73 and CML228. Minor QTL associated with variation in the flg22 ROS response was identified on chromosomes 1 and 4. Comparison of these results with data previously obtained for variation in QDR and the defense response in the same RIL population did not provide any evidence for a common genetic basis controlling variation in these traits.
鉴定了与玉米对两种微生物相关分子模式（MAMPs）反应变异相关的基因座。MAMP反应具有相关性。未发现MAMP反应与定量抗病性之间存在关联。微生物相关分子模式（MAMPs）是微生物中常见的高度保守分子，可被植物模式识别受体识别。识别会触发一系列反应，包括活性氧（ROS）和一氧化氮（NO）的产生以及防御相关基因的表达变化。在本研究中，我们使用两种经过充分研究的MAMPs（flg22和壳寡糖）挑战不同的玉米品系，以确定对这些MAMPs的反应水平是否存在差异，剖析该差异背后的遗传基础，并了解MAMP反应与定量抗病性（QDR）之间的关系。观察到由MAMPs触发的ROS、NO产生和防御基因表达水平的自然发生的定量变异。在源自玉米自交系B73和CML228的重组自交系（RIL）群体中，在2号染色体上鉴定出一个与对flg22和壳寡糖的ROS产生反应变异相关的主要数量性状基因座（QTL）。在1号和4号染色体上鉴定出与flg22 ROS反应变异相关的次要QTL。将这些结果与先前在同一RIL群体中获得的QDR变异和防御反应数据进行比较，没有提供任何证据表明存在控制这些性状变异的共同遗传基础。
