Zhang Yuxuan, Wang Fumin, Liu Ning, Yang Lin, Zhang Dabing
College of Life Science, South China Agricultural University, Guangzhou, People's Republic of China.
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian district, Beijing, 100193, People's Republic of China.
Arch Virol. 2017 Jul;162(7):2135-2139. doi: 10.1007/s00705-017-3297-1. Epub 2017 Mar 18.
We report the complete genome sequence of a new avastrovirus of goose-origin (FLX). The 7299-nt-long genome consisted of three overlapping open reading frames (ORFs) that were in different reading frames. Pairwise comparisons showed that the FLX genome was 59% identical to its closest relatives and that the levels of amino acid identity shared by FLX with other astroviruses did not exceed 54% in ORF1a, 66% in ORF1b, and 50% in ORF2, respectively. Phylogenetic analysis based on the amino acid sequence of the full-length ORF2 demonstrated that FLX was highly divergent from all other avastroviruses. At the amino acid level the complete capsid region of FLX shared genetic distances of 0.574-0.719 with three official avastrovirus species, suggesting that it can be classified as a member of a novel species in the genus Avastrovirus.
我们报告了一种新的鹅源星状病毒（FLX）的全基因组序列。该基因组长度为7299个核苷酸，由三个处于不同阅读框的重叠开放阅读框（ORF）组成。成对比较显示，FLX基因组与其最密切相关的病毒有59%的同一性，并且FLX与其他星状病毒在ORF1a、ORF1b和ORF2中共享的氨基酸同一性水平分别不超过54%、66%和50%。基于全长ORF2氨基酸序列的系统发育分析表明，FLX与所有其他禽星状病毒高度不同。在氨基酸水平上，FLX的完整衣壳区域与三种官方禽星状病毒物种的遗传距离为0.574 - 0.719，这表明它可被归类为禽星状病毒属中一个新物种的成员。
