1 Department of Obstetrics and Gynecology, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Reprod Sci. 2017 Sep;24(9):1280-1283. doi: 10.1177/1933719116682876. Epub 2016 Dec 14.
Recent studies have suggested that GnRH agonists (GnRHags) protect ovarian function following chemotherapy. Here, we study the effect of a combination of GnRH antagonist (GnRHan) and GnRHag for gonadal protection from gonadotoxic chemotherapy in adolescent female rats. Cycling Sprague Dawley rats were treated at adolescent age. Thirty female rats were randomized to 5 treatment groups (n = 6/group): (1) placebo, (2) cyclophosphamide (CPA) alone, (3) GnRHan followed by GnRHag with placebo, (4) GnRHan followed by GnRHag with CPA, and (5) GnRHag with CPA. The main outcome measure was live birth rate (LBR), and secondary measures included rat weight, ovarian volume, and follicles. Group 2 had decreased LBR compared to all other groups. Group 4 and 5 had LBR similar to placebo. There was no difference in the ovarian volume. The CPA-alone group had decreased number of antral follicles compared to control. These studies demonstrate that the combination of GnRHan and GnRHag and GnRHag alone preserved fertility in female adolescent rats following gonadotoxic chemotherapy treatment. The addition of a GnRHan to a GnRHag does not confer a greater protective effect.
最近的研究表明，促性腺激素释放激素激动剂（GnRHags）可在化疗后保护卵巢功能。在这里，我们研究了 GnRH 拮抗剂（GnRHan）和 GnRHag 联合用于保护青春期雌性大鼠性腺免受性腺毒性化疗的效果。对处于青春期的 Sprague Dawley 大鼠进行了周期性治疗。将 30 只雌性大鼠随机分为 5 个治疗组（每组 n = 6）：（1）安慰剂，（2）环磷酰胺（CPA）单独治疗，（3）GnRHan 加 GnRHag 联合安慰剂治疗，（4）GnRHan 加 GnRHag 联合 CPA 治疗，（5）单独用 GnRHag 加 CPA 治疗。主要观察指标是活产率（LBR），次要观察指标包括大鼠体重、卵巢体积和卵泡。与其他组相比，组 2 的 LBR 降低。组 4 和 5 的 LBR 与安慰剂相似。卵巢体积没有差异。与对照组相比，CPA 单独治疗组的窦卵泡数量减少。这些研究表明，GnRHan 和 GnRHag 联合应用以及 GnRHag 单独应用可在接受性腺毒性化疗的青春期雌性大鼠中保留生育能力。添加 GnRHan 到 GnRHag 中不会带来更大的保护作用。
