Yang Rongchang, Brice Belinda, Oskam Charlotte, Zhang Yujuan, Brigg Frances, Berryman Dave, Ryan Una
School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.
Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia, 6076, Australia.
Vet Parasitol. 2017 Apr 15;237:137-142. doi: 10.1016/j.vetpar.2017.01.027. Epub 2017 Feb 1.
The genus term Isospora is now applied specifically to parasites of birds, with the term Cystoisospora preferred for parasites which infect mammals. Isospora is a common parasitic coccidian in birds worldwide, especially in passerine birds, in which it can cause systemic coccidiosis. The complete mitochondrial genome sequences from two recently identified Isospora species; Isospora serinuse in a domestic canary and Isospora manorinae in a yellow-throated miner, were sequenced and compared with those of other closely related coccidian species. The complete mitochondrial genome sequence for Isospora serinuse is 6260bp in size and 6223bp for Isospora manorinae. The mitochondrial genomes of Isospora serinuse and Isospora manorinae include three protein-coding genes (COI, COIII and CytB), 19 LSU and 14 SSU rDNA fragments, including one newly identified putative LSU fragment in Isospora sp. The arrangement of coding regions in these two Isospora species were identical to that of available Isospora sp. and Eimeria spp. mitochondrial genomes and the start codon usage for protein coding genes was conservative. Phylogenetic analysis of the mt genome of the two Isospora species based on the three coding regions further support that the monophyletic nature of avian Isospora.
属名等孢球虫现在专门用于鸟类寄生虫，而对于感染哺乳动物的寄生虫，囊等孢球虫这个术语更受青睐。等孢球虫是全球鸟类中常见的寄生性球虫，尤其是在雀形目鸟类中，它可引发全身性球虫病。对最近鉴定出的两种等孢球虫物种的完整线粒体基因组序列进行了测序并与其他密切相关的球虫物种进行比较，这两种物种分别是家金丝雀体内的丝光椋鸟等孢球虫和黄喉矿工鸟体内的马诺里纳等孢球虫。丝光椋鸟等孢球虫的完整线粒体基因组序列大小为6260bp，马诺里纳等孢球虫为6223bp。丝光椋鸟等孢球虫和马诺里纳等孢球虫的线粒体基因组包括三个蛋白质编码基因（COI、COIII和CytB）、19个大亚基和14个小亚基rDNA片段，其中包括在等孢球虫属中一个新鉴定出的假定大亚基片段。这两种等孢球虫物种中编码区的排列与现有的等孢球虫属物种和艾美耳球虫属线粒体基因组相同，并且蛋白质编码基因的起始密码子使用情况较为保守。基于这三个编码区对这两种等孢球虫物种的线粒体基因组进行系统发育分析，进一步支持了鸟类等孢球虫的单系性质。
