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(顶复门:球虫纲)的线粒体全基因组序列

The Complete Mitochondrial Genome Sequence of (Apicomplexa: Coccidia).

作者信息

Shi Yubo, Fang Sufang, Gu Xiaolong, Hao Chengyu, Du Fangchen, Cui Ping, Tang Xinming

机构信息

College of Animal Science and Technology, Hebei North University, Zhangjiakou 075000, China.

Key Laboratory of Animal Biosafety Risk Prevention and Control (North), Key Laboratory of Veterinary Biological Products and Chemical Drugs of MARA, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

出版信息

Life (Basel). 2024 May 29;14(6):699. doi: 10.3390/life14060699.

Abstract

Rabbit coccidiosis is caused by infection with one or, more commonly, several species that parasitize the hepatobiliary ducts or intestinal epithelium of rabbits. Currently, there are eleven internationally recognized species of rabbit coccidia, with the complete mitochondrial (mt) genomes of six species commonly infecting rabbits having been sequenced and annotated. was initially discovered in 2011 and prompted a preliminary study on this species. Through traditional morphological analysis, was identified as a novel species of rabbit coccidia. To further validate this classification, we sequenced and annotated its mitochondrial genome. The complete mt genome of spans 6258 bp and comprises three cytochrome genes (), fourteen gene fragments for the large subunit (LSU) rRNA, and nine gene fragments for the small subunit (SSU) rRNA, lacking transfer RNA (tRNA) genes. Moreover, phylogenetic analysis of the mitochondrial genome sequence of revealed its clustering with six other species of rabbit coccidia into a monophyletic group. Additionally, and were grouped within the lineage lacking oocyst residuum, consistent with their morphological characteristics. Consistent with multiple molecular phylogenies, in this investigation, was further confirmed as a new species of rabbit coccidia. Our research findings are of great significance for the classification of coccidia and for coccidiosis prevention and control in rabbits.

摘要

兔球虫病是由一种或(更常见的是)几种寄生于兔肝胆管或肠道上皮的物种感染引起的。目前,国际上公认的兔球虫有11种,其中6种常见感染兔的球虫的完整线粒体(mt)基因组已被测序和注释。[该物种]于2011年首次被发现,并促使对该物种进行了初步研究。通过传统形态学分析,[该物种]被鉴定为兔球虫的一个新物种。为了进一步验证这一分类,我们对其线粒体基因组进行了测序和注释。[该物种]的完整线粒体基因组跨度为6258 bp,包含三个细胞色素基因([基因名称未给出])、14个大亚基(LSU)rRNA基因片段和9个小亚基(SSU)rRNA基因片段,缺少转运RNA(tRNA)基因。此外,对[该物种]线粒体基因组序列的系统发育分析表明,它与其他六种兔球虫聚集在一个单系类群中。此外,[该物种]和[另一物种名称未给出]被归为缺乏卵囊残体的谱系中,这与其形态特征一致。与多个分子系统发育结果一致,在本研究中,[该物种]被进一步确认为兔球虫的一个新物种。我们的研究结果对球虫的分类以及兔球虫病的预防和控制具有重要意义。

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