Kolasa-Trela Renata, Konieczynska Malgorzata, Bazanek Marta, Undas Anetta
Department of Diagnostic Medicine, John Paul II Hospital, Krakow, Poland.
Institute of Cardiology, Jagiellonian University Medical College, Krakow, Poland.
PLoS One. 2017 Mar 14;12(3):e0173787. doi: 10.1371/journal.pone.0173787. eCollection 2017.
We evaluated exercise-induced changes in the profile of circulating cytokines and growth factors in patients with AS.
We studied 32 consecutive asymptomatic moderate-to-severe AS patients and 32 age and sex-matched controls. Plasma levels of interleukin (IL)-6, IL-10, hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and transforming growth factor (TGF)-β were measured at 4 time points, i.e. at rest, at peak bicycle exercise, one hour and 24 hours after a symptom-limited exercise.
Exercise increased all the 5 markers in both groups (all p<0.0001). The maximum levels of all tested cytokines were higher in the AS group (all p<0.05) compared with controls. In AS patients the highest levels of VEGF, IL-6, and IL-10 were observed one hour after exercise, while in the control group at peak exercise. In both groups maximum TGF- β levels were observed one hour after exercise. HGF levels were higher at peak and one hour after test in the AS group (p = 0.0001), however the maximum value in AS was observed at peak while in controls after test. In both groups TGF-β was the only marker that remained increased 24 hours after exercise compared with the value at rest (p = 0.0001). The cytokines and growth factors showed no association with heart rate and the workload.
In asymptomatic patients with moderate-to-severe AS, exercise produces a different pattern of changes in circulating cytokines and growth factors, and maximum levels of all tested cytokines were significantly higher in AS patients compared with the control group.
我们评估了强直性脊柱炎（AS）患者运动诱导的循环细胞因子和生长因子谱的变化。
我们研究了32例连续的无症状中重度AS患者以及32例年龄和性别匹配的对照组。在4个时间点测量血浆白细胞介素（IL）-6、IL-10、肝细胞生长因子（HGF）、血管内皮生长因子（VEGF）和转化生长因子（TGF）-β水平，即静息时、自行车运动峰值时、症状限制运动后1小时和24小时。
运动使两组的所有5种标志物均升高（所有p<0.0001）。与对照组相比，AS组所有检测细胞因子的最高水平更高（所有p<0.05）。在AS患者中，运动后1小时观察到VEGF、IL-6和IL-10的最高水平，而在对照组中运动峰值时观察到最高水平。两组运动后1小时均观察到TGF-β的最高水平。AS组在测试峰值和测试后1小时HGF水平较高（p = 0.0001），然而AS组的最大值在峰值时观察到，而对照组在测试后观察到。与静息值相比，两组中TGF-β是运动后24小时仍保持升高的唯一标志物（p = 0.0001）。细胞因子和生长因子与心率和工作量无关联。
在无症状的中重度AS患者中，运动在循环细胞因子和生长因子中产生不同的变化模式，并且与对照组相比，AS患者中所有检测细胞因子的最高水平显著更高。
