抗人CD3抗体刺激后人CD3 T细胞中的微小RNA表达谱
MicroRNA expression profiles in human CD3 T cells following stimulation with anti-human CD3 antibodies.
作者信息
Sousa Isabel Garcia, do Almo Manuela Maragno, Simi Kelly Cristina Rodrigues, Bezerra Maryani Andressa Gomes, Andrade Rosângela Vieira, Maranhão Andréa Queiroz, Brigido Marcelo Macedo
机构信息
Molecular Pathology Graduation Program, Medicine Faculty, University of Brasilia, Brasilia, Brazil.
Molecular Biology Graduation Program, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.
出版信息
BMC Res Notes. 2017 Mar 14;10(1):124. doi: 10.1186/s13104-017-2442-y.
BACKGROUND
Anti-CD3 therapy can induce immunosuppression by several non mutually exclusive mechanisms that have been proposed to explain the therapeutic effect the administration anti-CD3 mAb, but its immunoregulatory mechanism is still not completely clear. In T cells, microRNAs (miRNAs) regulate several pathways, including those associated with immune tolerance. Here, we report changes in miRNA expression in T cells following treatment with anti-human CD3 antibodies. Peripheral blood mononuclear cells were cultured in the presence of the monoclonal antibody OKT3 or a recombinant fragment of humanized anti-CD3. Following these treatments, the expression profiles of 31 miRNA species were assessed in T cells using TaqMan arrays.
RESULTS
Eight of the tested miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b and miR-301a) were statistically significantly up- or down-regulated relative to untreated cells.
CONCLUSIONS
Stimulation of T cells with anti-human CD3 antibodies alters miRNA expression patterns, including of miRNA species associated with immune regulatory pathways.
背景
抗CD3疗法可通过多种并非相互排斥的机制诱导免疫抑制，这些机制已被提出用于解释抗CD3单克隆抗体给药后的治疗效果，但其免疫调节机制仍不完全清楚。在T细胞中，微小RNA（miRNA）调节多种途径，包括与免疫耐受相关的途径。在此，我们报告了用抗人CD3抗体处理后T细胞中miRNA表达的变化。外周血单个核细胞在单克隆抗体OKT3或人源化抗CD3重组片段存在的情况下进行培养。经过这些处理后，使用TaqMan芯片评估T细胞中31种miRNA的表达谱。
结果
与未处理的细胞相比，所测试的8种miRNA（miR-155、miR-21、miR-146a、miR-210、miR-17、miR-590-5p、miR-106b和miR-301a）在统计学上有显著的上调或下调。
结论
用人抗CD3抗体刺激T细胞会改变miRNA表达模式，包括与免疫调节途径相关的miRNA种类。
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