Carmo Andreia Moreira Dos Santos, Suzuki Rodrigo Buzinaro, Cabral Aline Diniz, Costa Renata Torres da, Massari Gabriela Pena, Riquena Michele Marcondes, Fracasso Helio Augusto Alves, Eterovic Andre, Marcili Arlei, Sperança Márcia Aparecida
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Bernardo do Campo, 09606-070 São Paulo, Brazil; Secretaria do Estado da Saúde do Estado de São Paulo, Instituto Adolfo Lutz, Centro de Laboratório Regional VIII, Santo André, 09040-160 São Paulo, Brazil.
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, São Bernardo do Campo, 09606-070 São Paulo, Brazil; Discipline of Parasitology, Marília Medical School, Marília, 17519-030, São Paulo, Brazil.
J Clin Virol. 2017 May;90:7-13. doi: 10.1016/j.jcv.2017.03.001. Epub 2017 Mar 6.
Dengue virus, represented by four distinct, genetically diverse serotypes, is the etiologic agent of asymptomatic to severe hemorrhagic diseases. The spatiotemporal dynamics of dengue serotypes and its association to specific diseases vary among the different regions worldwide. By 2007, and in São Paulo State, Brazil, dengue-case concentration in urban centers had changed to increased incidence in small- and medium-sized towns, the case of Marília.
The aim of this article was to distinguish dengue serotypes circulating during the 2007 Marília outbreak and define their association to demographic and hematological patient profiles, as well as the phylogenetic relationships among the different viruses.
PCR amplicons corresponding to the junction of capsid and dengue pre-membrane encoding genes, obtained from dengue serologically positive patients, were sequenced. Hematological and demographic data of patients with different Dengue serotypes were evaluated by univariate and bivariate statistics. Dengue PCR sequences were used in phylogenetic relationships analyzed for maximum parsimony.
Molecular typing confirmed co-circulation of the dengue serotypes 1 (DENV1) and 3 (DENV3), which presented divergent correlation patterns with regard to hematological descriptors. The increase in atypical lymphocytes, a likely indication of virus load, could be significantly associated to a decrease in leukocyte counts in the DENV3 group and platelet in the DENV1. Phylogenetic reconstitution revealed the introduction of DENV1 from northern Brazil and local divergence of DENV3 by either microevolution or viral introduction from other geographical regions or both.
Dengue dynamics showed regional molecular-epidemiologic specificity, which has important implications for introduction of vaccines, disease management, and transmission control.
登革病毒由四种不同的、基因多样的血清型代表，是无症状至严重出血性疾病的病原体。登革热血清型的时空动态及其与特定疾病的关联在全球不同地区有所不同。到2007年，在巴西圣保罗州，城市中心的登革热病例集中情况已转变为中小城镇发病率上升，以马利亚市为例。
本文旨在区分2007年马利亚市登革热疫情期间流行的登革热血清型，确定它们与患者人口统计学和血液学特征的关联，以及不同病毒之间的系统发育关系。
对从血清学确诊为登革热的患者中获得的、对应衣壳与登革热前膜编码基因交界处的PCR扩增子进行测序。通过单变量和双变量统计评估不同登革热血清型患者的血液学和人口统计学数据。登革热PCR序列用于分析最大简约法的系统发育关系。
分子分型证实登革热血清型1（DENV1）和3（DENV3）共同流行，它们在血液学指标方面呈现出不同的相关模式。非典型淋巴细胞增多可能表明病毒载量增加，这与DENV3组白细胞计数减少以及DENV1组血小板减少显著相关。系统发育重建显示DENV1从巴西北部引入，DENV3通过微进化或从其他地理区域引入病毒或两者兼而有之在当地发生分化。
登革热动态显示出区域分子流行病学特异性，这对疫苗引入、疾病管理和传播控制具有重要意义。
