Universidade Federal do ABC, Centro de Ciências Naturais e Humanas, São Bernardo do Campo, São Paulo, Brazil.
Instituto Adolfo Lutz, Centro de Imunologia, São Paulo, São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 2021 Mar 24;63:e17. doi: 10.1590/S1678-9946202163017. eCollection 2021.
Dengue virus, the etiological agent of dengue fever (DF) occurs in four genetically distinct serotypes (DENV1-4), being transmitted by female Aedes mosquitoes. DF incidence is increasing in Brazil, following vector dispersal, proliferation and DENV serotypes introduction, co-circulation and substitution. Medium- and small-sized cities in Sao Paulo State, such as Marilia (Midwest region), have been affected by huge epidemics. To understand the evolution of DENV epidemics in medium-sized cities, in this study a historical data on DENV incidence (2000-2015) in Marilia, was evaluated. Previous studies disclosed regional and specific DF outcomes associated with 2007 outbreak in that city, when co-circulating DENV1 and DENV3 presented different hematological profiles. In this study, characteristics of 2007 DENV epidemics were compared to the epidemiological, hematological and demographic outlines of the major outbreak of DENV1 in Marilia in 2015. DENV1 genetic diversity was assessed through capsid and pre-membrane junction encoding gene (CprM) sequencing. The results revealed circulation of DENV1 serotype from 2007 to 2015, with epidemics occurring every three-years until 2013 and then, increasing yearly. There were significant differences in hematological profiles of DENV1 patients between 2015 and 2007. CprM showed DENV1 genetic variability in 2015, contrasting with the unique sequence pattern in 2007. These results reinforce the regional and temporal characteristics of DENV epidemics that need local public health research to improve care for people and to limit the spread of new serotypes/genotypes to uninfected areas.
登革热病毒（Dengue virus）是登革热（DF）的病原体，存在于 4 种遗传上不同的血清型（DENV1-4）中，由雌性伊蚊传播。随着媒介的扩散、增殖以及 DENV 血清型的引入、共同循环和替代，巴西的 DF 发病率正在上升。圣保罗州的中、小城市，如马里利亚（中西部地区），已受到大规模疫情的影响。为了了解中型城市 DENV 疫情的演变，本研究评估了马里利亚 DENV 发病率的历史数据（2000-2015 年）。先前的研究揭示了与该市 2007 年暴发相关的区域性和特定性 DF 结果，当时共同循环的 DENV1 和 DENV3 呈现出不同的血液学特征。在这项研究中，将 2007 年 DENV 疫情的特征与 2015 年马里利亚 DENV1 主要暴发的流行病学、血液学和人口统计学概况进行了比较。通过对衣壳和前膜连接编码基因（CprM）进行测序，评估了 DENV1 的遗传多样性。结果显示，DENV1 血清型从 2007 年到 2015 年持续循环，疫情每三年发生一次，直到 2013 年，然后每年增加。2015 年和 2007 年 DENV1 患者的血液学特征存在显著差异。CprM 显示 2015 年 DENV1 的遗传变异性，与 2007 年的独特序列模式形成对比。这些结果强化了 DENV 疫情的区域性和时间性特征，需要进行当地公共卫生研究，以改善对患者的护理，并限制新血清型/基因型向未感染地区的传播。
