Zhang Man-Li, Jiang Yan-Fang, Wang Xin-Rui, Ding Li-Li, Wang Hong-Juan, Meng Qing-Qing, Gao Pu-Jun
Man-Li Zhang, Qing-Qing Meng, Department of Hepatology and Gastroenterology, The Second Part of First Hospital of Jilin University, Changchun 130021, Jilin Province, China.
World J Gastroenterol. 2017 Feb 28;23(8):1477-1488. doi: 10.3748/wjg.v23.i8.1477.
To evaluate the numbers of different subsets of monocytes and their associations with the values of clinical measures in mild acute pancreatitis (MAP) patients.
The study included one group of 13 healthy controls and another group of 24 patients with new-onset MAP. The numbers of different subsets of monocytes were examined in these two groups of subjects by flow cytometry. The concentrations of plasma interleukin (IL)-10 and IL-12 were determined by cytometric bead array. The acute physiology and chronic health evaluation (APACHE) II scores of individual patients were evaluated, and the levels of plasma C-reactive protein (CRP) as well as the activities of amylase and lipase were measured.
In comparison with that in the controls, significantly increased numbers of CD14+CD163-, CD14+CD163-MAC387+ M1 monocytes, but significantly reduced numbers of CD14+CD163+IL-10+ M2 monocytes were detected in the MAP patients ( < 0.01 or < 0.05). Furthermore, significantly higher levels of plasma IL-10 and IL-12 were observed in the MAP patients ( < 0.01 for all). More importantly, the levels of plasma CRP were positively correlated with the numbers of CD14+CD163- ( = 0.5009, = 0.0127) and CD14+CD163-MAC387+ ( = 0.5079, = 0.0113) M1 monocytes and CD14+CD163+CD115+ M2 monocytes ( = 0.4565, = 0.0249) in the patients. The APACHE II scores correlated with the numbers of CD14+CD163+CD115+ ( = 0.4581, = 0.0244) monocytes and the levels of plasma IL-10 ( = 0.4178, = 0.0422) in the MAP patients. However, there was no significant association among other measures tested in this population.
Increased numbers of CD14+CD163- and CD14+ CD163-MAC387+ monocytes may contribute to the pathogenesis of MAP, and increased numbers of CD14+CD163+CD115+ monocytes may be a biomarker for evaluating the severity of MAP.
评估轻度急性胰腺炎（MAP）患者单核细胞不同亚群的数量及其与临床指标值的相关性。
本研究包括一组13名健康对照者和另一组24名新发MAP患者。通过流式细胞术检测这两组受试者单核细胞不同亚群的数量。采用细胞计数珠阵列法测定血浆白细胞介素（IL）-10和IL-12的浓度。评估个体患者的急性生理与慢性健康状况评估（APACHE）II评分，并检测血浆C反应蛋白（CRP）水平以及淀粉酶和脂肪酶的活性。
与对照组相比，MAP患者中CD14+CD163-、CD14+CD163-MAC387+ M1单核细胞数量显著增加，但CD14+CD163+IL-10+ M2单核细胞数量显著减少（<0.01或<0.05）。此外，MAP患者血浆IL-10和IL-12水平显著更高（均<0.01）。更重要的是，患者血浆CRP水平与CD14+CD163-（r = 0.5009，P = 0.0127）、CD14+CD163-MAC387+（r = 0.5079，P = 0.0113）M1单核细胞以及CD14+CD163+CD115+ M2单核细胞数量（r = 0.4565，P = 0.0249）呈正相关。MAP患者的APACHE II评分与CD14+CD163+CD115+（r = 0.4581，P = 0.0244）单核细胞数量以及血浆IL-10水平（r = 0.4178，P = 0.0422）相关。然而，在该人群中测试的其他指标之间无显著相关性。
CD14+CD163-和CD14+CD163-MAC387+单核细胞数量增加可能参与MAP的发病机制，而CD14+CD163+CD115+单核细胞数量增加可能是评估MAP严重程度的生物标志物。
