6857National Institute of Environmental Health Sciences, National Toxicology Program, Cellular and Molecular Pathology Branch, Research Triangle Park, NC, USA.
Department of Clinical Sciences, North Carolina State University, Raleigh, NC, USA.
Vet Pathol. 2021 Sep;58(5):952-963. doi: 10.1177/03009858211023946. Epub 2021 Jul 1.
Evasion of the immune response is an integral part of the pathogenesis of glioma. In humans, important mechanisms of immune evasion include recruitment of regulatory T cells (Tregs) and polarization of macrophages toward an M2 phenotype. Canine glioma has a robust immune cell infiltrate that has not been extensively characterized. The purpose of this study was to determine the distribution of immune cells infiltrating spontaneous intracranial canine gliomas. Seventy-three formalin-fixed, paraffin-embedded tumor samples were evaluated using immunohistochemistry for CD3, forkhead box 3 (FOXP3), CD20, Iba1, calprotectin (Mac387), CD163, and indoleamine 2,3-dioxygenase (IDO). Immune cell infiltration was present in all tumors. Low-grade and high-grade gliomas significantly differed in the numbers of FoxP3+ cells, Mac387+ cells, and CD163+ cells ( = .006, .01, and .01, respectively). Considering all tumors, there was a significant increase in tumor area fraction of CD163 compared to Mac387 ( < .0001), and this ratio was greater in high-grade tumors than in low-grade tumors ( = .005). These data warrant further exploration into the roles of macrophage repolarization or Treg interference therapy in canine glioma.
免疫逃逸是神经胶质瘤发病机制的一个组成部分。在人类中，重要的免疫逃逸机制包括调节性 T 细胞（Treg）的募集和巨噬细胞向 M2 表型的极化。犬神经胶质瘤有丰富的免疫细胞浸润，但尚未得到广泛描述。本研究旨在确定浸润自发性颅内犬神经胶质瘤的免疫细胞的分布。使用免疫组织化学法检测 CD3、叉头框蛋白 3（FOXP3）、CD20、Iba1、钙卫蛋白（Mac387）、CD163 和吲哚胺 2,3-双加氧酶（IDO），对 73 例福尔马林固定、石蜡包埋的肿瘤样本进行评估。所有肿瘤均存在免疫细胞浸润。低级别和高级别神经胶质瘤的 FoxP3+细胞、Mac387+细胞和 CD163+细胞数量存在显著差异（=.006、.01 和.01）。考虑到所有肿瘤，与 Mac387 相比，CD163 的肿瘤区域分数显著增加（<.0001），且高级别肿瘤的比值高于低级别肿瘤（=.005）。这些数据表明，需要进一步探索巨噬细胞重极化或 Treg 干扰治疗在犬神经胶质瘤中的作用。
