Ma Yu, Ye Yao, Zhang Jin, Ruan Cheng-Chao, Gao Ping-Jin
State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Hypertension, Department of Hypertension, Ruijin Hospital and Shanghai Institute of Hypertension, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, Germany.
Medicine (Baltimore). 2019 Apr;98(14):e15080. doi: 10.1097/MD.0000000000015080.
Preeclampsia (PE) is characterized by hypertension and proteinuria. It affects about 5% to 8% of pregnancies and causes maternal and perinatal mortality and morbidity. The immune imbalance and excessive inflammatory response play vital roles in the pathogenesis of PE.In this study, we performed a case-control study to investigate the levels of cytokines, chemokines and adhesion molecules in serum and placenta of normal pregnant and PE women by Bio-Plex multiplex immunoassay and immunohistochemistry. In addition, we explored the phenotypes of monocyte and macrophage in peripheral blood and placentas in 2 groups by using flow cytometry analysis and immunohistochemistry.Our results show that pro-inflammatory factors, including interleukin-1β (IL-1β), IL-6, IL-7, IL-8, IL-17a, monocyte chemotactic protein 1 (MCP -1), and macrophage inflammatory protein 1β (MIP-1β) were significantly increased in serum of women with PE compared with controls. In addition, we detected that IL-1β, IL-6, and MCP-1 were also increased in placentas of women with PE. We further revealed that peripheral blood monocytes showed a pro-inflammatory M1-like phenotype in women with PE. Consistently, M1 macrophage infiltration was increased in placenta of women with PE compared to that of normal pregnant women.Our results demonstrated that immune imbalance promotes an inflammatory state during PE and it may be a potential therapeutic possibility for the management of PE.
子痫前期（PE）的特征是高血压和蛋白尿。它影响约5%至8%的妊娠，并导致孕产妇和围产期死亡及发病。免疫失衡和过度的炎症反应在PE的发病机制中起着至关重要的作用。在本研究中，我们进行了一项病例对照研究，通过生物芯片多重免疫测定和免疫组织化学来研究正常孕妇和PE患者血清及胎盘中细胞因子、趋化因子和黏附分子的水平。此外，我们通过流式细胞术分析和免疫组织化学来探究两组外周血和胎盘中单核细胞和巨噬细胞的表型。我们的结果表明，与对照组相比，PE患者血清中促炎因子，包括白细胞介素-1β（IL-1β）、IL-6、IL-7、IL-8、IL-17a、单核细胞趋化蛋白1（MCP -1）和巨噬细胞炎性蛋白1β（MIP-1β）显著增加。此外，我们检测到PE患者胎盘组织中IL-1β、IL-6和MCP-1也有所增加。我们进一步发现，PE患者外周血单核细胞呈现促炎的M1样表型。同样，与正常孕妇相比，PE患者胎盘组织中M1巨噬细胞浸润增加。我们的结果表明，免疫失衡在PE期间促进炎症状态，这可能是PE治疗管理的一种潜在治疗可能性。
