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透明质酸对暴露于乙醇的人皮肤成纤维细胞中粘着斑激酶活性的保护作用。

Protective influence of hyaluronic acid on focal adhesion kinase activity in human skin fibroblasts exposed to ethanol.

作者信息

Donejko Magdalena, Rysiak Edyta, Galicka Elżbieta, Terlikowski Robert, Głażewska Edyta Katarzyna, Przylipiak Andrzej

机构信息

Department of Esthetic Medicine.

Department of Medicinal Chemistry, Faculty of Pharmacy.

出版信息

Drug Des Devel Ther. 2017 Mar 6;11:669-676. doi: 10.2147/DDDT.S125843. eCollection 2017.

DOI:10.2147/DDDT.S125843
PMID:28293103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5345991/
Abstract

AIM

The aim of this study was to evaluate the effect of ethanol and hyaluronic acid (HA) on cell survival and apoptosis in cultured human skin fibroblasts. Regarding the mechanism of ethanol action on human skin fibroblasts, we investigated cell viability and apoptosis, expression of focal adhesion kinase (FAK), and the influence of HA on those processes.

MATERIALS AND METHODS

Studies were conducted in confluent human skin fibroblast cultures that were treated with 25 mM, 50 mM, and 100 mM ethanol or with ethanol and 500 µg/mL HA. Cell viability was examined using methyl thiazolyl tetrazolium (MTT) assay and NC-300 Nucleo-Counter. Imaging of the cells using a fluorescence microscope Pathway 855 was performed to measure FAK expression.

RESULTS

Depending on the dosage, ethanol decreased cell viability and activated the process of apoptosis in human skin fibroblasts. HA prevented the negative influence of ethanol on cell viability and prevented apoptosis. The analysis of fluorescence imaging using BD Pathway 855 High-Content Bioimager showed the inhibition of FAK migration to the cell nucleus, depending on the increasing concentration of ethanol.

CONCLUSION

This study proves that downregulation of signaling pathway of FAK is involved in ethanol-induced apoptosis in human skin fibroblasts. The work also indicates a protective influence of HA on FAK activity in human skin fibroblasts exposed to ethanol.

摘要

目的

本研究旨在评估乙醇和透明质酸（HA）对培养的人皮肤成纤维细胞存活和凋亡的影响。关于乙醇对人皮肤成纤维细胞的作用机制，我们研究了细胞活力和凋亡、粘着斑激酶（FAK）的表达，以及HA对这些过程的影响。

材料与方法

在汇合的人皮肤成纤维细胞培养物中进行研究，用25 mM、50 mM和100 mM乙醇或乙醇与500 µg/mL HA处理。使用甲基噻唑基四氮唑（MTT）法和NC-300细胞核计数器检测细胞活力。使用荧光显微镜Pathway 855对细胞进行成像，以测量FAK表达。

结果

根据剂量不同，乙醇会降低人皮肤成纤维细胞的活力并激活凋亡过程。HA可防止乙醇对细胞活力的负面影响并防止凋亡。使用BD Pathway 855高内涵生物成像仪对荧光成像进行分析显示，随着乙醇浓度的增加，FAK向细胞核的迁移受到抑制。

结论

本研究证明FAK信号通路的下调参与了乙醇诱导的人皮肤成纤维细胞凋亡。该研究还表明HA对暴露于乙醇的人皮肤成纤维细胞中的FAK活性具有保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/eefb725c99bf/dddt-11-669Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/7618de14c7f4/dddt-11-669Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/2f297d74e2ae/dddt-11-669Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/5b5e12becfab/dddt-11-669Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/eefb725c99bf/dddt-11-669Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/7618de14c7f4/dddt-11-669Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/2f297d74e2ae/dddt-11-669Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/5b5e12becfab/dddt-11-669Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53b8/5345991/eefb725c99bf/dddt-11-669Fig4.jpg

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